Lee Doyun, Lee Albert K
Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34141, Republic of Korea;
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147
Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.top087304. doi: 10.1101/pdb.top087304.
Intracellular recording is an essential technique for investigating cellular mechanisms underlying complex brain functions. Despite the high sensitivity of the technique to mechanical disturbances, intracellular recording has been applied to awake, behaving, and even freely moving, animals. Here we summarize recent advances in these methods and their application to the measurement and manipulation of membrane potential dynamics for understanding neuronal computations in behaving animals.
细胞内记录是研究复杂脑功能背后细胞机制的一项重要技术。尽管该技术对机械干扰高度敏感，但细胞内记录已应用于清醒、行为活动甚至自由移动的动物。在此，我们总结这些方法的最新进展及其在测量和操纵膜电位动力学以理解行为动物神经元计算方面的应用。
