Cao Ting-Ting, Zhang Yu-Qing
Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, RM702-2303, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou, 215123, People's Republic of China.
Amino Acids. 2017 Jun;49(6):1029-1039. doi: 10.1007/s00726-017-2396-3. Epub 2017 Apr 3.
Cell culture and cryopreservation are necessary for clinical therapy and cells storage. The addition of 10% (v/v) foetal bovine serum (FBS) to basal culture media has been common practice and is one of the most widely used methods. FBS media added with 10% DMSO (dimethyl sulfoxide) have also been used for cryopreservation cells. Ideally, FBS should be avoided because of high cost and bio-safety. Silk sericin has been used as a serum substitute and an additive due to its good hydrophilicity and biological safety. This article summarizes a few details about the processing of sericin and its application as a serum substitute or an additive for cell culture and cryopreservation media. Sericin can be a potential novel serum substitute or an additive for cell culture and cryopreservation media.
细胞培养和冷冻保存对于临床治疗和细胞储存至关重要。在基础培养基中添加10%（v/v）胎牛血清（FBS）是常见做法，也是使用最广泛的方法之一。添加10%二甲基亚砜（DMSO）的FBS培养基也已用于细胞冷冻保存。理想情况下，由于成本高和生物安全性问题，应避免使用FBS。丝胶蛋白因其良好的亲水性和生物安全性，已被用作血清替代品和添加剂。本文总结了丝胶蛋白的制备过程及其作为细胞培养和冷冻保存培养基的血清替代品或添加剂的应用的一些细节。丝胶蛋白可能是一种潜在的新型细胞培养和冷冻保存培养基的血清替代品或添加剂。
