Kim Soo In, Jeon Ga Yeong, Kim Se Eun, Choe Seung Ho, Kim Seung Jae, Seo Jin Sol, Kang Tae Woong, Song Jeong Eun, Khang Gilson
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk54896, Republic of Korea.
Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk54896, Republic of Korea.
ACS Omega. 2022 Nov 2;7(45):41331-41340. doi: 10.1021/acsomega.2c05113. eCollection 2022 Nov 15.
The damage to retinal pigment epithelium (RPE) cells can lead to vision loss and permanent blindness. Therefore, an effective therapeutic strategy has emerged to replace damaged cells through RPE cell delivery. In this study, we fabricated injectable gellan gum (GG)/silk sericin (SS) hydrogels as a cell carrier by blending GG and SS. To determine the appropriate concentration of SS for human RPE ARPE-19, 0, 0.05, 0.1, and 0.5% (w/v) of SS solution were blended in 1% (w/v) GG solution (GG/SS 0%, GG/SS 0.05%, GG/SS 0.1%, and GG/SS 0.5%, respectively). The physical and chemical properties were measured through Fourier-transform infrared spectroscopy, scanning electron microscopy, mass swelling, and weight loss. Also, viscosity, injection force, and compressive tests were used to evaluate mechanical characteristics. Cell proliferation and differentiation of ARPE-19 were evaluated using quantitative dsDNA analysis and real-time polymerase chain reaction, respectively. The addition of SS gave GG/SS hydrogels a compressive strength similar to that of natural RPE tissue, which may well support the growth of RPE and enhance cell proliferation and differentiation. In particular, the GG/SS 0.5% hydrogel showed the most similar compressive strength (about 10 kPa) and exhibited the highest gene expression related to ARPE-19 cell proliferation. These results indicate that GG/SS 0.5% hydrogels can be a promising biomaterial for cell delivery in retina tissue engineering.
视网膜色素上皮（RPE）细胞的损伤会导致视力丧失和永久性失明。因此，一种有效的治疗策略应运而生，即通过RPE细胞递送替代受损细胞。在本研究中，我们通过将结冷胶（GG）和丝胶蛋白（SS）混合，制备了可注射的GG/SS水凝胶作为细胞载体。为了确定适合人RPE ARPE-19细胞的SS浓度，将0%、0.05%、0.1%和0.5%（w/v）的SS溶液分别与1%（w/v）的GG溶液混合（分别为GG/SS 0%、GG/SS 0.05%、GG/SS 0.1%和GG/SS 0.5%）。通过傅里叶变换红外光谱、扫描电子显微镜、质量膨胀和失重测量其物理和化学性质。此外，还使用粘度、注射力和压缩试验来评估力学特性。分别使用定量双链DNA分析和实时聚合酶链反应评估ARPE-19细胞的增殖和分化。SS的添加使GG/SS水凝胶具有与天然RPE组织相似的抗压强度，这很可能支持RPE的生长并增强细胞增殖和分化。特别是，GG/SS 0.5%水凝胶表现出最相似的抗压强度（约10 kPa），并且与ARPE-19细胞增殖相关的基因表达最高。这些结果表明，GG/SS 0.5%水凝胶可能是视网膜组织工程中一种有前景的细胞递送生物材料。
