通过脉冲场凝胶电泳和16S rRNA基因测序对共生奈瑟菌属菌株进行基因分型。
Genotyping of commensal Neisseria spp strains by pulsed-field gel electrophoresis and 16S rRNA gene sequencing.
作者信息
Mechergui Arij, Achour Wafa, Ben Hassen Assia
机构信息
The National Bone Marrow Transplant Centre, Tunis, Tunisia.
Faculty of Medicine of Tunis, UR12ES02, Tunis, Tunisia.
出版信息
J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22208. Epub 2017 Apr 4.
BACKGROUND
We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis.
METHODS
Identification to the genus was done using conventional methods and API NH (bio-Mérieux ). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI.
RESULTS
Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty-three different PFGE patterns were found among the tested strains.
CONCLUSION
We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.
背景
我们研究了46株共生奈瑟菌菌株中16S rRNA基因一级序列的多样性，并与脉冲场凝胶电泳（PFGE）分析相比较，评估了将该方法用作分子分型工具的实用性。
方法
使用常规方法和API NH（生物梅里埃公司）进行属的鉴定。种水平的鉴定基于16S rRNA基因测序。使用斯皮I酶进行PFGE分析。
结果
在20株微黄奈瑟菌、2株干燥奈瑟菌、5株猕猴奈瑟菌和19株粘膜炎奈瑟菌临床分离株中分别发现了14种、2种、3种和14种16S rRNA序列类型。在测试菌株中发现了43种不同的PFGE图谱。
结论
我们证明了16S rRNA基因间存在高度多样性，这在PFGE分析中得到了体现。
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