Kooliyottil Rinu, Dandurand Louise-Marie, Knudsen Guy R
Department of Plant, Soil and Entomological Sciences, University of Idaho, Moscow, Idaho.
Soil and Land Resources Division, University of Idaho, Moscow, Idaho.
J Basic Microbiol. 2017 May;57(5):386-392. doi: 10.1002/jobm.201600683. Epub 2017 Apr 4.
Seven filamentous fungal species were isolated from individual eggs of Globodera pallida cysts collected from infested fields in Shelley Idaho, USA and identified as Chaetomium globosum, Fusarium oxysporum, Fusarium solani, Fusarium tricinctum, Microdochium bolleyi, Purpureocillium lilacinum, and Plectosphaerella cucumerina. Their ability to reduce infection by G. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-ROCs). All fungi reduced G. pallida infection in potato, but greatest reduction was observed with C. globosum at an average reduction of 76%. Further non-destructive methods were developed to rapidly assess biological control potential of putative fungal strains by staining the infectious second stage juveniles of G. pallida with the live fluorescent stain PKH26. In comparisons between the standard, invasive acid fuchsin method and use of the live stain PKH26, no significant difference in infection level of G. pallida was observed whether roots were stained with PKH26 or acid fuchsin. For both methods, a similar reduction (77% for acid fuchsin, and 78% for PKH26 stain) in invasion of infectious stage of G. pallida was observed when potato plants were inoculated with C. globosum compared to non-inoculated potato.
从美国爱达荷州雪莱受侵染田地采集的苍白球孢囊线虫单个卵中分离出七种丝状真菌物种，分别鉴定为球毛壳菌、尖孢镰刀菌、茄形镰刀菌、三线镰刀菌、博莱微dochium菌、淡紫紫孢菌和黄瓜盘多毛孢菌。在简单、可重复的微根际室（micro-ROCs）中评估了它们在植物体内减少苍白球孢囊线虫感染的能力。所有真菌都能减少马铃薯中苍白球孢囊线虫的感染，但球毛壳菌的减少效果最为显著，平均减少率为76%。还开发了进一步的非破坏性方法，通过用活荧光染料PKH26对苍白球孢囊线虫感染性第二阶段幼虫进行染色，快速评估推定真菌菌株的生物防治潜力。在标准的侵入性酸性品红方法与使用活染料PKH26的比较中，无论用PKH26还是酸性品红对根进行染色，苍白球孢囊线虫的感染水平均未观察到显著差异。对于这两种方法，与未接种马铃薯相比，用球毛壳菌接种马铃薯时，苍白球孢囊线虫感染阶段的侵入减少情况相似（酸性品红为77%，PKH26染色为78%）。
