Department of Stomatology, Lanzhou General Hospital, Lanzhou Military Area Command of Chinese PLA, Lanzhou, Gansu, China.
State Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Int Endod J. 2018 Jan;51(1):26-40. doi: 10.1111/iej.12777. Epub 2017 May 4.
To evaluate in vitro the physicochemical properties, cytotoxicity and calcium phosphate nucleation of an experimental light-curable pulp capping material composed of a resin with antibacterial monomer (MAE-DB) and Portland cement (PC).
The experimental material was prepared by mixing PC with a resin containing MAE-DB at a 2 : 1 ratio. Cured pure resin containing MAE-DB served as control resin. ProRoot MTA and Dycal served as commercial controls. The depth of cure, degree of monomer conversion, water absorption and solubility of dry samples, calcium release, alkalinizing activity, calcium phosphate nucleation and the cytotoxicity of materials were evaluated. Statistical analysis was carried out using anova followed by Tukey's HSD test (equal variance assumed) or Tamhane test (equal variance not assumed) and independent-samples t-tests.
The experimental material had a cure depth of 1.19 mm, and the mean degree of monomer conversion was 70.93% immediately post-cure and 88.75% at 24 h post-cure. The water absorption of the experimental material was between those of MTA and Dycal, and its solubility was significantly less (P < 0.05) than that of Dycal and higher than that of MTA. The experimental material exhibited continuous calcium release and an alkalinizing power between those of MTA and Dycal throughout the test period. Freshly set experimental material, control resin and all 24-h set materials had acceptable cytotoxicity. The experimental material, MTA and Dycal all exhibited the formation of apatite precipitates after immersion in phosphate-buffered saline.
The experimental material possessed adequate physicochemical properties, low cytotoxicity and good calcium phosphate nucleation.
评价一种由含抗菌单体（MAE-DB）树脂和波特兰水泥（PC）组成的实验性光固化牙髓覆盖材料的理化性能、细胞毒性和磷酸钙成核能力。
将 PC 与含 MAE-DB 的树脂以 2：1 的比例混合制备实验材料。固化的纯含 MAE-DB 树脂作为对照树脂。ProRoot MTA 和 Dycal 作为商业对照。评估干样的深度固化、单体转化率、吸水率和溶解度、钙释放、碱化活性、磷酸钙成核和材料的细胞毒性。采用方差分析（方差齐性假设）或 Tamhane 检验（方差不齐性假设）以及独立样本 t 检验对数据进行统计分析。
实验材料的固化深度为 1.19mm，即刻固化后单体转化率的平均值为 70.93%，24h 后为 88.75%。实验材料的吸水率介于 MTA 和 Dycal 之间，其溶解度显著低于（P<0.05）Dycal，高于 MTA。实验材料在整个测试期间持续释放钙并具有介于 MTA 和 Dycal 之间的碱化能力。新鲜的实验材料、对照树脂和所有 24h 固化的材料均具有可接受的细胞毒性。新鲜设置的实验材料、对照树脂和所有 24 小时设置的材料在磷酸盐缓冲盐水中浸泡后均能形成磷灰石沉淀。
实验材料具有足够的理化性能、低细胞毒性和良好的磷酸钙成核能力。
