Reilly J G, Thomas C A, Sen A
Biochim Biophys Acta. 1982 Apr 26;697(1):53-9. doi: 10.1016/0167-4781(82)90044-6.
Methylation of DNA in normal mouse cultured 3T3 cells and in their virally or chemically transformed derivatives was studied. DNA methylation was studied by restriction with HpaII, MspI, or HpaII plus MspI. DNA from the chemically transformed cells was cleaved about twice as often with HpaII than was the DNA of normal and virally transformed cells. Digests with MspI and HpaII plus MspI were identical in all cell lines studied. Densitometry of the restriction patterns allowed an estimate of total DNA methylation from the weight average lengths. The chemically transformed cell line showed 25% reduction in methylation compared to the other cell lines. Southern blot hybridization using satellite DNA showed that these sequences followed a pattern of modification similar to that of total DNA.
研究了正常小鼠培养的3T3细胞及其病毒或化学转化衍生物中DNA的甲基化情况。通过用HpaII、MspI或HpaII加MspI进行酶切来研究DNA甲基化。与正常和病毒转化细胞的DNA相比，化学转化细胞的DNA被HpaII切割的频率大约是其两倍。在所研究的所有细胞系中，用MspI以及HpaII加MspI进行的酶切结果是相同的。对酶切图谱进行光密度测定，可根据平均长度估计总DNA甲基化水平。与其他细胞系相比，化学转化细胞系的甲基化水平降低了25%。使用卫星DNA进行的Southern印迹杂交表明，这些序列的修饰模式与总DNA相似。
