• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

B7-H3在小鼠附睾头、体和尾中的不同表达。

Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.

作者信息

Li Kai, Wei Xuedong, Zhang Guangbo, Li Miao, Zhang Xuefeng, Zhou Chenhao, Hou Jianquan, Yuan Hexing

机构信息

Department of Urology, The First Affiliated Hospital of Soochow University, NO.188 Shizi Road, Suzhou, 215006, Jiangsu, China.

The Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, NO. 708 Renmin Road, Suzhou, 215006, China.

出版信息

BMC Urol. 2017 Apr 4;17(1):23. doi: 10.1186/s12894-017-0215-5.

DOI:10.1186/s12894-017-0215-5
PMID:28376772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5379651/
Abstract

BACKGROUND

B7-H3, a member of the B7 family of the Ig superfamily of proteins, has been detected in the epididymis, which is a storage organ related to sperm maturation. However, the characteristics of its expression in different regions of the epididymis remain unknown. Our aim was to investigate the expression of B7-H3 in the caput, corpus, and cauda of the epididymis.

METHODS

We extracted epididymis specimens from adult male C57BL/6 mice. The expression of B7-H3 was then measured with immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and western blotting.

RESULTS

B7-H3 protein was predominantly detected on the membrane and in the cytoplasm of the principal cells in the epididymis. Moreover, the level of B7-H3 in the corpus of the mouse epididymis was significantly higher than that in the caput (p < 0.05) or the cauda of the epididymis (P < 0.05). However, there was no remarkable difference in the level of B7-H3 between the caput and the cauda (p > 0.05).

CONCLUSIONS

The caput, corpus, and cauda of the mouse epididymis all expressed B7-H3 protein. However, the levels of B7-H3 were different in the three regions of the epididymis.

摘要

背景

B7 - H3是免疫球蛋白超家族B7家族的成员之一，已在附睾中被检测到，附睾是一个与精子成熟相关的储存器官。然而，其在附睾不同区域的表达特征仍不清楚。我们的目的是研究B7 - H3在附睾头、体、尾中的表达情况。

方法

我们从成年雄性C57BL/6小鼠中提取附睾标本。然后用免疫组织化学、酶联免疫吸附测定（ELISA）和蛋白质印迹法检测B7 - H3的表达。

结果

在附睾主细胞的细胞膜和细胞质中主要检测到B7 - H3蛋白。此外，小鼠附睾体中B7 - H3的水平显著高于附睾头（p < 0.05）或附睾尾（P < 0.05）。然而，附睾头和附睾尾之间B7 - H3的水平没有显著差异（p > 0.05）。

结论

小鼠附睾的头、体、尾均表达B7 - H3蛋白。然而，B7 - H3在附睾的三个区域中的水平不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/e611d6acc412/12894_2017_215_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/60ef88352b29/12894_2017_215_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/be6dd56f2a22/12894_2017_215_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/001462fba8b4/12894_2017_215_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/e611d6acc412/12894_2017_215_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/60ef88352b29/12894_2017_215_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/be6dd56f2a22/12894_2017_215_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/001462fba8b4/12894_2017_215_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5534/5379651/e611d6acc412/12894_2017_215_Fig4_HTML.jpg

相似文献

1
Different expression of B7-H3 in the caput, corpus, and cauda of the epididymis in mouse.B7-H3在小鼠附睾头、体和尾中的不同表达。
BMC Urol. 2017 Apr 4;17(1):23. doi: 10.1186/s12894-017-0215-5.
2
Region-specific gene expression in the epididymis of Yak.牦牛附睾组织的基因表达具有区域特异性。
Theriogenology. 2019 Nov;139:132-146. doi: 10.1016/j.theriogenology.2019.08.006. Epub 2019 Aug 5.
3
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions.人类附睾上皮细胞的表达谱揭示了附睾头、体和尾区域的功能多样性。
Mol Hum Reprod. 2016 Feb;22(2):69-82. doi: 10.1093/molehr/gav066. Epub 2015 Nov 26.
4
Comparative study of boar sperm coming from the caput, corpus, and cauda regions of the epididymis.来自附睾头、体、尾区域的公猪精子的比较研究。
J Androl. 1995 Mar-Apr;16(2):175-88.
5
Isolation, immunolocalization, and sperm-association of three proteins of 18, 25, and 29 kilodaltons secreted by the mouse epididymis.小鼠附睾分泌的三种分子量分别为18、25和29千道尔顿的蛋白质的分离、免疫定位及与精子的结合
Biol Reprod. 1992 May;46(5):747-66. doi: 10.1095/biolreprod46.5.747.
6
Expression and location of taurine transporters and channels in the epididymis of infertile c-ros receptor tyrosine kinase-deficient and fertile heterozygous mice.不育的c-ros受体酪氨酸激酶缺陷型小鼠和可育的杂合子小鼠附睾中牛磺酸转运体和通道的表达及定位
Mol Reprod Dev. 2003 Feb;64(2):144-51. doi: 10.1002/mrd.10250.
7
Segment- and cell-specific expression of D-type cyclins in the postnatal mouse epididymis.D型细胞周期蛋白在出生后小鼠附睾中的节段和细胞特异性表达。
Gene Expr Patterns. 2012 Mar-Apr;12(3-4):136-44. doi: 10.1016/j.gep.2012.01.003. Epub 2012 Jan 24.
8
Electron microscopic immunolocalization of the 18 and 29 kilodalton secretory proteins in the mouse epididymis: evidence for differential uptake by clear cells.小鼠附睾中18千道尔顿和29千道尔顿分泌蛋白的电子显微镜免疫定位：透明细胞差异摄取的证据。
Microsc Res Tech. 1995 Jan 1;30(1):24-36. doi: 10.1002/jemt.1070300103.
9
Developmental expression of sulfated glycoprotein-2 in the epididymis of the rat.大鼠附睾中硫酸化糖蛋白-2的发育表达
Anat Rec. 1994 Nov;240(3):327-44. doi: 10.1002/ar.1092400306.
10
[Analysis of DNA content of spermatogenic cells in the adult human testis and epididymis by flow cytometry].
Zhonghua Nan Ke Xue. 2002;8(5):335-7.

引用本文的文献

1
The Role of Mononuclear Phagocytes in the Testes and Epididymis.单核吞噬细胞在睾丸和附睾中的作用。
Int J Mol Sci. 2022 Dec 20;24(1):53. doi: 10.3390/ijms24010053.

本文引用的文献

1
Epididymal protein markers and fertility.附睾蛋白标志物与生育能力。
Anim Reprod Sci. 2016 Jun;169:76-87. doi: 10.1016/j.anireprosci.2016.02.034. Epub 2016 Mar 2.
2
Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.CD14+单核细胞中B7-H3的过表达与肾细胞癌进展相关。
Med Oncol. 2014 Dec;31(12):349. doi: 10.1007/s12032-014-0349-1. Epub 2014 Nov 22.
3
B7-H3 expression in breast cancer and upregulation of VEGF through gene silence.B7-H3 在乳腺癌中的表达及通过基因沉默上调 VEGF。
Onco Targets Ther. 2014 Oct 29;7:1979-86. doi: 10.2147/OTT.S63424. eCollection 2014.
4
B7-H3 promoted sperm motility in humans.B7-H3 可增强人类精子的运动能力。
Urology. 2014 Feb;83(2):324-30. doi: 10.1016/j.urology.2013.07.078. Epub 2013 Nov 15.
5
B7-H3-mediated tumor immunology: Friend or foe?B7-H3 介导的肿瘤免疫学：是敌是友？
Int J Cancer. 2014 Jun 15;134(12):2764-71. doi: 10.1002/ijc.28474. Epub 2013 Sep 30.
6
Stimulation of B7-H3 (CD276) directs the differentiation of human marrow stromal cells to osteoblasts.B7-H3（CD276）的刺激可引导人骨髓基质细胞向成骨细胞分化。
Immunobiology. 2011 Dec;216(12):1311-7. doi: 10.1016/j.imbio.2011.05.013. Epub 2011 May 30.
7
B7-H3 over expression in prostate cancer promotes tumor cell progression.B7-H3 在前列腺癌中的过表达促进肿瘤细胞进展。
J Urol. 2011 Sep;186(3):1093-9. doi: 10.1016/j.juro.2011.04.103. Epub 2011 Jul 23.
8
The epididymal transcriptome and proteome provide some insights into new epididymal regulations.附睾转录组和蛋白质组为新的附睾调节机制提供了一些见解。
J Androl. 2011 Nov-Dec;32(6):651-64. doi: 10.2164/jandrol.111.013086. Epub 2011 Jul 15.
9
Detection and quantitation of soluble B7-H3 in expressed prostatic secretions: a novel marker in patients with chronic prostatitis.检测和定量分析分泌型前列腺液中的可溶性 B7-H3：慢性前列腺炎患者的一种新型标志物。
J Urol. 2011 Feb;185(2):532-7. doi: 10.1016/j.juro.2010.09.104. Epub 2010 Dec 18.
10
Identification of luminal and secreted proteins in bull epididymis.鉴定公牛附睾中的腔蛋白和分泌蛋白。
J Proteomics. 2011 Jan 1;74(1):59-78. doi: 10.1016/j.jprot.2010.07.013. Epub 2010 Aug 6.