高酚含量部位所致豨莶草的抗高尿酸血症、抗炎和镇痛作用
Anti-Hyperuricemic, Anti-Inflammatory and Analgesic Effects of Siegesbeckia orientalis L. Resulting from the Fraction with High Phenolic Content.
作者信息
Nguyen Thuy Duong, Thuong Phuong Thien, Hwang In Hyun, Hoang Thi Kim Huyen, Nguyen Minh Khoi, Nguyen Hoang Anh, Na MinKyun
机构信息
Department of Pharmacology, Hanoi University of Pharmacy, 13 Le Thanh Tong, Hoan Kiem, Hanoi, Vietnam.
Department of Pharmaceutical Analysis and Standardization, National Institute of Medicinal Materials, 3B Quang Trung, Hoan Kiem, Hanoi, Vietnam.
出版信息
BMC Complement Altern Med. 2017 Apr 4;17(1):191. doi: 10.1186/s12906-017-1698-z.
BACKGROUND
The medicinal plant Siegesbeckia orientalis L. has been commonly used for the treatment of acute arthritis, rheumatism, and gout in Vietnam. However, pharmacological research of this plant associated with gout has not been reported. Anti-hyperuricemic and anti-inflammatory effects were evaluated and observed for the crude ethanol extract (CEE) of S. orientalis. Retention of these biological properties was found in a n-butanol-soluble fraction (BuOH fr.) of the extract, and therefore further biological and chemical investigations were undertaken on the BuOH fr. to support the medical relevance of this plant.
METHODS
The aerial part of S. orientalis was obtained in the mountainous region of Vietnam. The crude ethanol extract (CEE) and its BuOH fr. were prepared from the plant materials. Anti-hyperuricemic activities of the CEE and BuOH fr. were tested in vivo using the model oxonate-induced hyperuricemia rats through determination of serum uric acid levels and inhibitory effects on xanthine oxidase (XO) in the rat liver. Anti-inflammatory activities of the BuOH fr. were also evaluated in vivo using carrageenan-induced paw edema and urate-induced synovitis in rats. Active components of the BuOH fr. were characterized by comparison of HPLC retention time (t ) and spectroscopic data (UV, H-NMR) with those of reference compounds.
RESULTS
The CEE of S. orientalis displayed anti-hyperuricemic activity, and the BuOH fr. was found to be the most active portion of the extract. Further in vivo studies on this fraction showed 31.4% decrease of serum uric acid levels, 32.7% inhibition of xanthine oxidase (XO), 30.4% reduction of paw edema volume, symptomatic relief in urate-induced synovitis and significant analgesic effect at the dose of 120 mg/kg, as compared to the corresponding values of the control groups. Chemical analysis of the BuOH fr. revealed high phenolic content, identified as caffeic acid analogues and flavonones.
CONCLUSIONS
This study suggested that anti-hyperuricemic and anti-inflammatory mechanism of S. orientalis is related to XO inhibitory effect of the phenolic components. Our findings support the use of this plant as the treatment of gout and other inflammatory diseases.
背景
药用植物东方豨莶在越南通常用于治疗急性关节炎、风湿和痛风。然而，尚未见关于该植物与痛风相关的药理学研究报道。对东方豨莶的粗乙醇提取物（CEE）进行了抗高尿酸血症和抗炎作用的评估与观察。在提取物的正丁醇可溶部分（BuOH fr.）中发现保留了这些生物学特性，因此对BuOH fr.进行了进一步的生物学和化学研究，以支持该植物的医学相关性。
方法
东方豨莶的地上部分采自越南山区。从植物材料中制备粗乙醇提取物（CEE）及其BuOH fr.。通过测定血清尿酸水平和对大鼠肝脏中黄嘌呤氧化酶（XO）的抑制作用，在体内测试CEE和BuOH fr.的抗高尿酸血症活性。还通过角叉菜胶诱导的大鼠足爪肿胀和尿酸盐诱导的大鼠滑膜炎在体内评估BuOH fr.的抗炎活性。通过将BuOH fr.的高效液相色谱保留时间（t）和光谱数据（紫外、氢核磁共振）与参考化合物的进行比较，对其活性成分进行表征。
结果
东方豨莶的CEE表现出抗高尿酸血症活性，且BuOH fr.被发现是提取物中活性最强的部分。对该部分的进一步体内研究表明，与对照组的相应值相比，在120mg/kg剂量下，血清尿酸水平降低31.4%，黄嘌呤氧化酶（XO）抑制32.7%，足爪肿胀体积减少30.4%，尿酸盐诱导的滑膜炎症状缓解且有显著镇痛作用。对BuOH fr.的化学分析显示其酚类含量高，鉴定为咖啡酸类似物和黄酮酮。
结论
本研究表明东方豨莶的抗高尿酸血症和抗炎机制与酚类成分对XO的抑制作用有关。我们的研究结果支持将该植物用于治疗痛风和其他炎症性疾病。
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