Miron Talia, Wilchek Meir
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
Anal Biochem. 2017 Jun 15;527:1-3. doi: 10.1016/j.ab.2017.03.026. Epub 2017 Apr 1.
A colorimetric method for determining cyanuric chloride (CC) and for monitoring its polysaccharide gel activation, before and after ligand binding, was developed. The method is based on the reaction of CC or its activated gel with pyridine and barbituric acid or dimethylbarbituric acid. The product formed yields a purple red color with λ max at 595 nm, and an E value of approximately 300,000 after 1 h at room temperature. Due to its high sensitivity, this method can detect traces of CC (1 μM) under the above conditions. The method is fast, reliable and devoid of any side reactions.
开发了一种比色法，用于测定三聚氯氰（CC）以及监测其在配体结合前后多糖凝胶的活化情况。该方法基于CC或其活化凝胶与吡啶和巴比妥酸或二甲基巴比妥酸的反应。所形成的产物在595nm处产生紫红色，室温下1小时后吸光系数（E值）约为300,000。由于其高灵敏度，该方法在上述条件下可检测到痕量的CC（1μM）。该方法快速、可靠且无任何副反应。
