Ministry of Education Key Laboratory of Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 May 15;215:307-312. doi: 10.1016/j.saa.2019.02.109. Epub 2019 Mar 1.
In this work, photoactivated oxidase mimetics was prepared by copolymerizing dicyandiamide with barbituric acid (BA) and characterized by X-ray diffraction pattern, Fourier transformed infrared spectrum, X-ray photoelectron spectroscopy, transmission electron microscopy, photoluminescence spectrum, diffuse reflectance spectrum. Experimental results and density functional theory calculation indicated that the substitution of nitrogen atoms by carbon atoms in tri-s-triazine structure due to the copolymerization of BA enhanced visible light absorption and weakened the barrier of photocarrier transfer. In the presence of visible light and oxygen, 3, 3', 5, 5'-tetramethylbenzidine was oxidized under the catalysis of photoactivated oxidase mimetics to produce a green colored product, which could be reduced by glutathione (GSH). Therefore, a facile method based on the photoactivated oxidase mimetic has been developed for colorimetric detection of GSH. The linear range for GSH was ranged from 2.0 to 50.0 μmol L (R = 0.998) with the detection limit of 1.4 μmol L. The proposed method was applied to detect the cellular GSH with satisfactory results.
在这项工作中，通过将双氰胺与巴比妥酸（BA）共聚制备了光激活氧化酶模拟物，并通过 X 射线衍射图、傅里叶变换红外光谱、X 射线光电子能谱、透射电子显微镜、光致发光光谱、漫反射光谱进行了表征。实验结果和密度泛函理论计算表明，由于 BA 的共聚，三嗪结构中氮原子被碳原子取代，增强了可见光吸收，减弱了光载流子转移的势垒。在可见光和氧气存在下，光激活氧化酶模拟物催化 3,3',5,5'-四甲基联苯胺氧化生成绿色产物，该产物可被谷胱甘肽（GSH）还原。因此，基于光激活氧化酶模拟物开发了一种用于 GSH 比色检测的简便方法。GSH 的线性范围为 2.0 至 50.0 μmol L（R = 0.998），检测限为 1.4 μmol L。该方法已成功应用于细胞内 GSH 的检测。
