School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou, 225009, China.
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Fudan University, Shanghai, 200438, China.
Sci Rep. 2017 Apr 4;7(1):635. doi: 10.1038/s41598-017-00279-5.
Corythucha ciliata (Say), an invasive pest originating from North America, causes severe damage on sycamore trees. However, little is known about the population genetics and evolutionary forces underlying the invasiveness of this important pest. In the present study, we use three mitochondrial genes (COI, ND1 and ND5) and nine microsatellite markers to investigate the population genetics of C. ciliata and retrace its spread through China. The results suggest a low level of genetic diversity in Chinese and European populations of C. ciliata. Our results indicate that populations of C. ciliata have obvious genetic structure, and genetic differentiation is not caused by geographic isolation. In median-joining networks, we observed a higher frequency of shared haplotypes in groups 1 and 3. Based on gene flow and approximate Bayesian computation analyses, we discovered that C. ciliata first invaded the east coast of China and subsequently moved inland. Demographic analysis suggested that populations of C. ciliata in China may have undergone a recent bottleneck effect. Finally, our results suggest that population structure, high gene flow and environmental conditions have favored the broad invasiveness of this important pest.
Corythucha ciliata (Say)，一种源自北美的入侵性害虫，对槭树造成严重破坏。然而，对于这种重要害虫的入侵性背后的种群遗传学和进化力量知之甚少。在本研究中，我们使用了三个线粒体基因（COI、ND1 和 ND5）和九个微卫星标记来研究 C. ciliata 的种群遗传学，并追溯它在中国的传播途径。结果表明，中国和欧洲的 C. ciliata 种群遗传多样性水平较低。我们的结果表明，C. ciliata 种群具有明显的遗传结构，遗传分化不是由地理隔离引起的。在中位数连接网络中，我们观察到组 1 和组 3 中共享单倍型的频率更高。基于基因流和近似贝叶斯计算分析，我们发现 C. ciliata 首先入侵了中国的东海岸，随后向内陆移动。种群动态分析表明，中国的 C. ciliata 种群可能经历了近期的瓶颈效应。最后，我们的结果表明，种群结构、高基因流和环境条件有利于这种重要害虫的广泛入侵。
