Boulay Anne-Cécile, Saubaméa Bruno, Adam Nicolas, Chasseigneaux Stéphanie, Mazaré Noémie, Gilbert Alice, Bahin Mathieu, Bastianelli Leïla, Blugeon Corinne, Perrin Sandrine, Pouch Juliette, Ducos Bertrand, Le Crom Stéphane, Genovesio Auguste, Chrétien Fabrice, Declèves Xavier, Laplanche Jean-Louis, Cohen-Salmon Martine
Neuroglial Interactions in Cerebral Physiopathology/Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique CNRS, UMR 7241/Institut National de la Santé et de la Recherche Médicale INSERM, U1050/University Pierre et Marie Curie UPMC, ED, No. 158/MEMOLIFE Laboratory of Excellence and PSL Research University, Paris, France; Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité, Paris, France.
Variabilité des réponses aux psychotropes, INSERM U1144/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Paris Diderot/Université Sorbonne Paris Cité, Paris, France; Cellular and Molecular Imaging Facility, INSERM US25/CNRS UMS 3612/Faculté de Pharmacie de Paris/Université Paris Descartes/Université Sorbonne Paris Cité, Paris, France.
Cell Discov. 2017 Mar 28;3:17005. doi: 10.1038/celldisc.2017.5. eCollection 2017.
Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.
星形胶质细胞发出长突起，其末端在血管表面形成终足，并调节血管功能以及血管界面处的内环境稳态。迄今为止，这些功能背后的星形胶质细胞机制尚未得到充分研究。在这里，我们证明了一部分信使核糖核酸分布在星形胶质细胞终足中。在这个转录组中，我们鉴定出一组与核糖体结合的信使核糖核酸，即终足转录组，其主要编码分泌蛋白和膜蛋白。我们在星形胶质细胞终足中检测到新生蛋白质合成。最后，我们确定了星形胶质细胞血管周围突起和终足中存在光滑和粗糙内质网以及高尔基体，提示膜蛋白和分泌蛋白在局部成熟。这些结果首次证明蛋白质合成发生在星形胶质细胞血管周围远端突起中，这可能维持它们在血管界面处的结构和功能极化。
