对神经元中mRNA动态的单分子见解。
Single-molecule insights into mRNA dynamics in neurons.
作者信息
Buxbaum Adina R, Yoon Young J, Singer Robert H, Park Hye Yoon
机构信息
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA; Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Trends Cell Biol. 2015 Aug;25(8):468-75. doi: 10.1016/j.tcb.2015.05.005. Epub 2015 Jun 4.
Abstract
Targeting of mRNAs to neuronal dendrites and axons plays an integral role in intracellular signaling, development, and synaptic plasticity. Single-molecule imaging of mRNAs in neurons and brain tissue has led to enhanced understanding of mRNA dynamics. Here we discuss aspects of mRNA regulation as revealed by single-molecule detection, which has led to quantitative analyses of mRNA diversity, localization, transport, and translation. These exciting new discoveries propel our understanding of the life of an mRNA in a neuron and how its activity is regulated at the single-molecule level.
摘要
将信使核糖核酸（mRNA）靶向运输至神经元树突和轴突在细胞内信号传导、发育及突触可塑性方面发挥着不可或缺的作用。对神经元和脑组织中mRNA进行单分子成像已增进了我们对mRNA动态变化的理解。在此，我们将探讨单分子检测所揭示的mRNA调控的各个方面，这些研究成果已促成了对mRNA多样性、定位、运输及翻译的定量分析。这些令人振奋的新发现推动了我们对神经元中mRNA生命周期及其在单分子水平上的活性调控方式的理解。
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