Hess R G, Coulibaly C O, Greiser-Wilke I, Moennig V, Liess B
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Vet Microbiol. 1988 Apr;16(4):315-21. doi: 10.1016/0378-1135(88)90013-2.
A collection of 90 field isolates of hog cholera virus (HCV) was used to test the specificity of four hybridoma cell lines secreting monoclonal antibodies against pestiviruses. Reaction of virus isolates and monoclonal antibodies was controlled by an indirect immunofluorescence assay (IFA). Two monoclonal antibodies which had been generated against HC virus strain "Alfort 187" were reactive only with HCV field isolates and an HCV reference strain but not with bovine viral diarrhoea virus (BVDV) reference strains. Two other monoclonal antibodies (generated against BVDV, strain NADL) reacted only with BVDV reference strains but not with HCV field isolates, although with 3 of these strains focal reactions involving only a few cells were detected. The ability to discriminate between both viruses is a diagnostic need which may be fulfilled by these monoclonal antibodies.
使用90株猪瘟病毒（HCV）野外分离株来测试4株分泌抗瘟病毒单克隆抗体的杂交瘤细胞系的特异性。病毒分离株与单克隆抗体的反应通过间接免疫荧光试验（IFA）进行控制。针对HC病毒株“Alfort 187”产生的两种单克隆抗体仅与HCV野外分离株和一株HCV参考株发生反应，而不与牛病毒性腹泻病毒（BVDV）参考株发生反应。另外两种单克隆抗体（针对BVDV株NADL产生）仅与BVDV参考株发生反应，而不与HCV野外分离株发生反应，不过在其中3株HCV野外分离株中检测到仅涉及少数细胞的局灶性反应。区分这两种病毒的能力是一项诊断需求，而这些单克隆抗体可能满足这一需求。
