Edwards S, Sands J J, Harkness J W
Central Veterinary Laboratory, Weybridge, Surrey, U.K.
Arch Virol. 1988;102(3-4):197-206. doi: 10.1007/BF01310825.
Monoclonal antibodies were prepared against bovine virus diarrhoea virus and hog cholera virus. They were used to test 101 field isolates of ruminant pestivirus in a simple binding assay using an indirect immunoperoxidase label on fixed cell cultures. The monoclonals were divided into three panels: (1) pestivirus group specific, (2) hog cholera specific, (3) selectively reactive with ruminant pestiviruses. The reaction patterns with panel 3 were analyzed by a computer spreadsheet to determine the percentage match with seven reference patterns. Field viruses could be divided into two main groups. Group A consisted of 73 (87%) of the 84 bovine isolates and three (19%) of the 16 ovine, and was reactive with 40% or more of panel 3. Group B showed only limited reactivity with panel 3 and comprised 13/16 (81%) of the ovine (border disease) isolates together with the remaining 11 (13%) bovine viruses.
制备了抗牛病毒性腹泻病毒和猪瘟病毒的单克隆抗体。在固定细胞培养物上使用间接免疫过氧化物酶标记的简单结合试验中，用它们来检测101株反刍动物瘟病毒的田间分离株。这些单克隆抗体被分为三组：（1）瘟病毒属特异性，（2）猪瘟特异性，（3）与反刍动物瘟病毒选择性反应。用计算机电子表格分析与第3组的反应模式，以确定与七种参考模式的匹配百分比。田间病毒可分为两个主要组。A组由84株牛分离株中的73株（87%）和16株羊分离株中的3株（19%）组成，与第3组的40%或更多反应。B组与第3组仅表现出有限的反应性，包括16株羊（边界病）分离株中的13株（81%）以及其余11株（13%）牛病毒。
