Dubuisson J, Thiry E, Thalasso F, Bublot M, Pastoret P P
Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
Vet Microbiol. 1988 Apr;16(4):339-49. doi: 10.1016/0378-1135(88)90015-6.
Bovid herpesvirus-4 (BHV-4) isolates V.Test and LVR140, isolated from genital disease, respectively, in bull and in cow, and the reference strains Movar 33/63 and DN599 were compared by several methods: cross-serological relationship studied by indirect immunofluorescence; kinetics of intracellular and extracellular viral production; comparison of the mean plaque size; restriction analysis of viral DNA with restriction enzymes EcoRI, BamHI and HindIII. BHV-4 strains were serologically identical and the kinetics of viral production were very similar. Comparison of the mean plaque size allowed classification into 3 classes (Class I, Movar33/63; Class II, LVR140; Class III, V.Test and DN599) and restriction analysis of viral DNA revealed clear differences between the electrophoretic patterns of the four BHV-4 strains. The differentiation between BHV-4 strains can therefore be achieved by a biological method (mean plaque size) and by restriction analysis. The two genital isolates are easily differentiated by the two methods.
牛疱疹病毒4型（BHV - 4）毒株V.Test和LVR140分别从公牛和母牛的生殖器疾病中分离得到，将其与参考毒株Movar 33/63和DN599通过多种方法进行比较：采用间接免疫荧光研究交叉血清学关系；研究细胞内和细胞外病毒产生的动力学；比较平均蚀斑大小；用限制性内切酶EcoRI、BamHI和HindIII对病毒DNA进行限制性分析。BHV - 4毒株血清学上相同，病毒产生动力学非常相似。平均蚀斑大小的比较可将其分为3类（I类，Movar33/63；II类，LVR140；III类，V.Test和DN599），病毒DNA的限制性分析显示这4种BHV - 4毒株的电泳图谱存在明显差异。因此，BHV - 4毒株之间的区分可通过生物学方法（平均蚀斑大小）和限制性分析来实现。这两种生殖器分离株通过这两种方法很容易区分。
