Bublot M, Dubuisson J, Van Bressem M F, Danyi S, Pastoret P P, Thiry E
Virology Department, Faculty of Veterinary Medicine, University of Liège, Brussels, Belgium.
J Gen Virol. 1991 Mar;72 ( Pt 3):715-9. doi: 10.1099/0022-1317-72-3-715.
Herpesvirus aotus type 2 (HVA-2) was isolated from a culture of kidney cells from a healthy owl monkey (Aotus trivirgatus). Bovine herpesvirus type 4 (BHV-4) is frequently isolated from diseased and even healthy cattle and occasionally from sheep, wild ruminants and cats. The two viruses are related antigenically, as was revealed by an indirect fluorescent antibody test using polyclonal antisera from experimentally infected rabbits or monoclonal antibodies raised against six BHV-4 proteins, three of which were glycosylated. The genome structures of the two viruses consist of a unique central sequence flanked at both ends by G + C-rich tandem repeats. Restriction maps (produced using EcoRI, BamHI and HindIII) of these two viruses were nearly identical but the unique sequence of the HVA-2 genome possessed two additional BamHI sites. Four genomic regions of variable size were detected, two located in the unique part, one in the repetitive part and one in the left junction between the unique and the repeated part of the genome; these slight variations were similar to those observed between various BHV-4 isolates. These results suggest that HVA-2 and BHV-4 belong to the same virus species; HVA-2 could be either a BHV-4 contaminant of owl monkey kidney cell cultures or an isolate from an owl monkey accidentally infected with BHV-4.
2型猴疱疹病毒（HVA - 2）是从一只健康夜猴（三带夜猴）的肾细胞培养物中分离出来的。4型牛疱疹病毒（BHV - 4）经常从患病甚至健康的牛中分离出来，偶尔也从绵羊、野生反刍动物和猫中分离出来。通过使用来自实验感染兔子的多克隆抗血清或针对六种BHV - 4蛋白（其中三种是糖基化的）产生的单克隆抗体进行间接荧光抗体试验，发现这两种病毒在抗原上相关。这两种病毒的基因组结构由一个独特的中央序列组成，两端侧翼是富含G + C的串联重复序列。这两种病毒的限制性图谱（使用EcoRI、BamHI和HindIII产生）几乎相同，但HVA - 2基因组的独特序列拥有两个额外的BamHI位点。检测到四个大小可变的基因组区域，两个位于独特部分，一个位于重复部分，一个位于基因组独特部分和重复部分之间的左侧连接处；这些微小差异与在各种BHV - 4分离株之间观察到的差异相似。这些结果表明，HVA - 2和BHV - 4属于同一病毒种；HVA - 2可能是夜猴肾细胞培养物中的BHV - 4污染物，或者是一只意外感染BHV - 4的夜猴的分离株。
