Storz J, Ehlers B, Todd W J, Ludwig H
J Gen Virol. 1984 Apr;65 ( Pt 4):697-706. doi: 10.1099/0022-1317-65-4-697.
Biological properties and restriction enzyme patterns of the slowly replicating herpesviruses isolated from cattle affected with different diseases in North America and Europe were analysed. These virus isolates induced identical plaques that developed within 7 to 9 days in bovine foetal spleen cells and within 5 days in actively growing Georgia bovine kidney cells. These virus isolates were found to be antigenically related when tested in the indirect immunofluorescence test, and antigenic relationships with bovine herpesvirus 1 (BHV-1), BHV-2, BHV-3 or BHV-6 were not detected. The genomes of these strains were shown to have virtually identical cleavage sites when treated with restriction enzymes EcoRI, BamHI, SstII, SphI and HindIII. The resulting restriction enzyme patterns differed strikingly from those of BHV-1, BHV-2, BHV-3 and BHV-6. Because the herpesviruses tested become enveloped on the nuclear as well as on endoplasmic membranes, a process through which they induce cytoplasmic vesicles filled with enveloped viral particles, and because of the unique cytoplasmic inclusions that are induced, we classify them tentatively as bovine cytomegaloviruses.
对从北美和欧洲患有不同疾病的牛身上分离出的缓慢复制型疱疹病毒的生物学特性和限制性内切酶图谱进行了分析。这些病毒分离株在牛胎儿脾细胞中7至9天内形成相同的噬斑，在活跃生长的佐治亚牛肾细胞中5天内形成相同的噬斑。在间接免疫荧光试验中检测发现，这些病毒分离株具有抗原相关性，未检测到与牛疱疹病毒1型（BHV-1）、BHV-2、BHV-3或BHV-6的抗原关系。用限制性内切酶EcoRI、BamHI、SstII、SphI和HindIII处理时，这些毒株的基因组显示出几乎相同的切割位点。所得的限制性内切酶图谱与BHV-1、BHV-2、BHV-3和BHV-6的图谱明显不同。由于所测试的疱疹病毒在核膜和内质膜上均被包膜，在此过程中它们诱导充满包膜病毒颗粒的细胞质囊泡，并且由于诱导产生独特的细胞质内含物，我们将它们初步归类为牛巨细胞病毒。
