Grünig H M, Lebek G
Institute of Hygiene and Medical Microbiology, University of Berne, Switzerland.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Mar;267(4):485-94.
20 hly plasmids of different origin and source and 8 recombinant plasmids containing either chromosomally or plasmid encoded hly determinants were characterized with emphasis on their haemolytic activity. Remarkable differences among the hly genes tested were found in their haemolytic activity and in the regulation of the haemolytic activity by the extracellular iron concentration. Under iron-limiting growth conditions the haemolysin secretion encoded by 6 hly plasmids, but not by the remaining 14 hly plasmids and the chromosomal hly genes, was significantly induced. The 6 plasmids all derived from human isolates, belonged to the incompatibility groups FIV and FVI and showed similar restriction patterns. Two hly determinants, encoding an inducible haemolysin secretion, cloned from plasmids isolated in Berne and Paris, respectively, showed identical restriction maps, indicating a common origin. Compared to the hly determinant of a plasmid encoding a noninducible haemolysin secretion, distinct differences, mainly in the flanking regions, were observed.
对20种不同来源和出处的hly质粒以及8种含有染色体或质粒编码的hly决定簇的重组质粒进行了表征，重点是它们的溶血活性。在所测试的hly基因之间，在溶血活性以及细胞外铁浓度对溶血活性的调节方面发现了显著差异。在铁限制生长条件下，6种hly质粒编码的溶血素分泌被显著诱导，而其余14种hly质粒和染色体hly基因编码的则未被诱导。这6种质粒均源自人类分离株，属于不相容群FIV和FVI，且显示出相似的限制性图谱。分别从伯尔尼和巴黎分离的质粒中克隆的两个编码可诱导溶血素分泌的hly决定簇，显示出相同的限制性图谱，表明它们有共同的起源。与编码不可诱导溶血素分泌的质粒的hly决定簇相比，观察到主要在侧翼区域存在明显差异。
