Grünig H M, Rutschi D, Schoch C, Lebek G
Institute of Hygiene and Medical Microbiology, University of Berne, Switzerland.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Aug;266(1-2):231-8. doi: 10.1016/s0176-6724(87)80036-6.
The haemolytic activity encoded by thirteen hly-plasmids of different origin and sources was examined as a function of the Fe3+-concentration in E. coli fur+ and E. coli fur- strains, respectively. In E. coli fur+ the relatively low haemolytic activity of five hly-plasmids isolated in Berne and one isolated in Paris was increased significantly under iron-limiting growth conditions. Contrastingly, in E. coli fur- strains containing the same plasmids, a considerably higher amount of secreted haemolysin was detected. This activity could not be further increased by limiting the extracellular iron concentration. Seven other hly-plasmids expressed similar and non-inducible amounts of secreted haemolysin in both E. coli fur+ and E. coli fur- strains. These results indicate that the extracellular haemolytic activity encoded by certain hly-plasmids was controlled by the chromosomally encoded fur gene.
分别检测了来自不同来源的13种hly质粒编码的溶血活性，该活性是大肠杆菌fur +和大肠杆菌fur-菌株中Fe3+浓度的函数。在大肠杆菌fur +中，在伯尔尼分离的5种hly质粒和在巴黎分离的1种hly质粒的相对较低的溶血活性在铁限制生长条件下显著增加。相反，在含有相同质粒的大肠杆菌fur-菌株中，检测到大量分泌的溶血素。通过限制细胞外铁浓度，这种活性不能进一步增加。其他7种hly质粒在大肠杆菌fur +和大肠杆菌fur-菌株中表达相似且不可诱导的分泌溶血素量。这些结果表明，某些hly质粒编码的细胞外溶血活性受染色体编码的fur基因控制。
