Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena &Jena University Hospital, Jena, Germany.
Department of Physiology, University of Pennsylvania, Philadelphia, USA.
Sci Rep. 2017 Apr 5;7:46003. doi: 10.1038/srep46003.
Photonic experiments are of key importance in life sciences but light-induced side effects are serious confounding factors. Here we introduce roNa2, an engineered voltage-gated Na channel harboring a selenocysteine in its inactivation motif, as a non-photonic, sensitive, gateable, and reversible sensor for membrane-delimited reactive species. roNa2 allows for the assessment of chemical modification induced in fluorescence microscopy settings with high sensitivity and time resolution and it demonstrates the usefulness of ion channels as highly sensitive reporters of membrane processes.
在生命科学中，光子实验至关重要，但光诱导的副作用是严重的混杂因素。在这里，我们引入 roNa2，这是一种经过工程改造的电压门控钠离子通道，其失活基序中含有硒代半胱氨酸，可作为一种非光子、敏感、可门控和可逆的膜限域活性物质传感器。roNa2 允许在荧光显微镜环境中评估高灵敏度和时间分辨率下诱导的化学修饰，它证明了离子通道作为膜过程的高灵敏度报告器的有用性。
