Park Kyoung Jin, Suh Won Se, Subedi Lalita, Kim Sun Yeou, Choi Sang Un, Lee Kang Ro
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University.
Chem Pharm Bull (Tokyo). 2017;65(4):359-364. doi: 10.1248/cpb.c16-00804.
Phytochemical investigation of the twigs of Syringa oblata var. diatata led to the isolation of two new secoiridoid glucosides, dilatioside A-B (1-2), along with thirteen known ones (3-15). The structures were determined by spectroscopic methods including one and two dimensional (1- and 2D-) NMR techniques, high resolution (HR)-FAB-MS, and chemical methods. The isolated compounds (1-15) were tested for the induction of nerve growth factor (NGF) secretion in a C6 rat glioma cell line and their cytotoxicity against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, HCT15) in vitro using a sulforhodamine B bioassay. Compounds 5, 7, 8, 10, and 14 were found to induce upregulation of NGF secretion without causing significant cell toxicity.
对紫丁香变种（Syringa oblata var. diatata）嫩枝进行植物化学研究，从中分离出两种新的裂环环烯醚萜苷，即扩张苷A - B（1 - 2），以及十三种已知化合物（3 - 15）。通过包括一维和二维（1D - 和2D -）核磁共振技术、高分辨率（HR）- FAB - MS在内的光谱方法以及化学方法确定了其结构。使用磺酰罗丹明B生物测定法，对分离得到的化合物（1 - 15）在C6大鼠胶质瘤细胞系中诱导神经生长因子（NGF）分泌的能力以及它们对四种人类癌细胞系（A549、SK - OV - 3、SK - MEL - 2、HCT15）的体外细胞毒性进行了测试。发现化合物5、7、8、10和14可诱导NGF分泌上调，且不会引起明显的细胞毒性。
