Suh Won Se, Kwon Oh Kil, Lee Tae Hyun, Subedi Lalita, Kim Sun Yeou, Lee Kang Ro
Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University.
Gachon Institute of Pharmaceutical Science, Gachon University.
Chem Pharm Bull (Tokyo). 2018;66(1):78-83. doi: 10.1248/cpb.c17-00720.
Two new secoiridoid glycosides, obtusifolisides A and B (1, 2), together with 7 known secoiridoid glycosides (3-9) were isolated from the twigs of Ligustrum obtusifolium. The chemical structures of new compounds were determined by a spectroscopic data analysis, including one and two dimensional (1D-, 2D)-NMR, High resolution-MS, and experiments involving chemical reactions. The isolated secoiridoid glycosides were evaluated for their anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated BV-2 murine microglia cells. Compounds 2, 5, 6, 8, and 9 significantly reduced the production of nitric oxide (NO), with IC values of 5.45, 11.17, 14.62, 15.45, and 14.96 µM, respectively. None of the compounds were toxic to the cells. Additionally, we evaluated the neuroprotective effects of compounds 1-9 on nerve growth factor (NGF) induction in a C6 rat glioma cell line. Compounds 2 and 6 upregulated NGF secretion to 155.56±7.16%, and 139.35±11.65%, respectively, without significant cell toxicity.
从钝叶女贞的嫩枝中分离出两种新的裂环环烯醚萜苷，即钝叶女贞苷A和B（1, 2），以及7种已知的裂环环烯醚萜苷（3 - 9）。通过光谱数据分析，包括一维和二维（1D - 2D）核磁共振、高分辨率质谱以及化学反应实验，确定了新化合物的化学结构。对分离得到的裂环环烯醚萜苷在脂多糖（LPS）刺激的BV - 2小鼠小胶质细胞中的抗炎作用进行了评估。化合物2、5、6、8和9显著降低了一氧化氮（NO）的产生，IC值分别为5.45、11.17、14.62、15.45和14.96 μM。所有化合物对细胞均无毒性。此外，我们评估了化合物1 - 9对C6大鼠胶质瘤细胞系中神经生长因子（NGF）诱导的神经保护作用。化合物2和6分别将NGF分泌上调至155.56±7.16%和139.35±11.65%，且对细胞无明显毒性。
