使用基于过氧化物酶的氧化还原探针监测酵母线粒体：氧气和葡萄糖可用性的影响。
Monitoring yeast mitochondria with peroxiredoxin-based redox probes: the influence of oxygen and glucose availability.
作者信息
Pastor-Flores Daniel, Becker Katja, Dick Tobias P
机构信息
Division of Redox Regulation, DKFZ-ZMBH Alliance , German Cancer Research Center (DKFZ) , Im Neuenheimer Feld 280, 69120 Heidelberg , Germany.
Biochemistry and Molecular Biology , Heinrich-Buff-Ring 26-32, Justus Liebig University , 35392 Giessen , Germany.
出版信息
Interface Focus. 2017 Apr 6;7(2):20160143. doi: 10.1098/rsfs.2016.0143.
Mitochondrially generated oxidants are believed to play important roles in both physiology and pathophysiology. Therefore, it is of significant interest to better understand the metabolic conditions leading to enhanced mitochondrial oxidant generation. Here, we investigate the influence of oxygen and glucose availability on the redox state of peroxiredoxin-based redox probes, expressed in the cytosol and mitochondrial matrix of yeast cells. We observe that the redox state of peroxiredoxin probes reflects the balance between dioxygen-dependent peroxide generation and glucose-dependent generation of reducing equivalents. The oxidative pentose phosphate pathway appears to be the dominant source of NADPH in the system under study.
线粒体产生的氧化剂被认为在生理和病理生理过程中都发挥着重要作用。因此，更好地理解导致线粒体氧化剂生成增加的代谢条件具有重大意义。在这里，我们研究了氧气和葡萄糖可用性对酵母细胞胞质溶胶和线粒体基质中表达的基于过氧化物酶的氧化还原探针氧化还原状态的影响。我们观察到，过氧化物酶探针的氧化还原状态反映了依赖于双氧的过氧化物生成与依赖于葡萄糖的还原当量生成之间的平衡。在本研究系统中，氧化戊糖磷酸途径似乎是NADPH的主要来源。
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