Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia.
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
Int J Mol Sci. 2020 Oct 31;21(21):8164. doi: 10.3390/ijms21218164.
Redox reactions are of high fundamental and practical interest since they are involved in both normal physiology and the pathogenesis of various diseases. However, this area of research has always been a relatively problematic field in the context of analytical approaches, mostly because of the unstable nature of the compounds that are measured. Genetically encoded sensors allow for the registration of highly reactive molecules in real-time mode and, therefore, they began a new era in redox biology. Their strongest points manifest most brightly in in vivo experiments and pave the way for the non-invasive investigation of biochemical pathways that proceed in organisms from different systematic groups. In the first part of the review, we briefly describe the redox sensors that were used in vivo as well as summarize the model systems to which they were applied. Next, we thoroughly discuss the biological results obtained in these studies in regard to animals, plants, as well as unicellular eukaryotes and prokaryotes. We hope that this work reflects the amazing power of this technology and can serve as a useful guide for biologists and chemists who work in the field of redox processes.
氧化还原反应具有很高的基础和实际意义，因为它们既涉及正常生理学，也涉及各种疾病的发病机制。然而，在分析方法的背景下，这个研究领域一直是一个相对有问题的领域，主要是因为所测量的化合物的不稳定性。遗传编码传感器允许实时记录高反应性分子，因此，它们开创了氧化还原生物学的新时代。它们的最强点在体内实验中表现得最为明显，并为非侵入性研究不同系统群的生物体中进行的生化途径铺平了道路。在综述的第一部分，我们简要描述了体内使用的氧化还原传感器，并总结了它们应用的模型系统。接下来，我们深入讨论了在这些研究中在动物、植物以及单细胞真核生物和原核生物中获得的生物学结果。我们希望这项工作反映了这项技术的惊人力量，并能为从事氧化还原过程领域的生物学家和化学家提供有用的指导。
