Keppens S, De Wulf H
Afdeling Biochemie, Campus Gasthuisberg, Katholieke Universiteit Leuven, Belgium.
FEBS Lett. 1988 Jun 6;233(1):47-50. doi: 10.1016/0014-5793(88)81353-x.
We have used the technique of short-term infusion with digitonin to obtain hepatocytes originating either from the periportal or the perivenous zone of the liver acinus [(1985) Biochem. J. 229, 221-226]. Total glycogen phosphorylase content and sensitivity to cyclic AMP-dependent and calcium-mediated glycogenolytic agonists were very similar for both cell sub-populations and did not differ from the values obtained for control cells. We conclude therefore that there is an apparent absence of metabolic zonation as far as receptor-mediated glycogenolysis and glycogenolytic potency is concerned.
我们采用了用洋地黄皂苷进行短期灌注的技术，以获得源自肝腺泡门静脉周围或肝静脉周围区域的肝细胞[（1985年）《生物化学杂志》229卷，221 - 226页]。对于这两个细胞亚群，总糖原磷酸化酶含量以及对环磷酸腺苷依赖性和钙介导的糖原分解激动剂的敏感性非常相似，且与对照细胞所获得的值没有差异。因此我们得出结论，就受体介导的糖原分解和糖原分解能力而言，明显不存在代谢分区现象。
