Akintunde Jacob K, Abubakar Opeyemi K
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Drug Metab Pers Ther. 2017 May 24;32(2):97-107. doi: 10.1515/dmpt-2016-0044.
Antipsychotic drugs could be nephrotoxic in schizophrenia patients.
The present study investigated the protective effect of oil from black seed on kidney dysfunctions using several biological approaches in adult rats. The animals were divided into six groups (n=10): normal control rats; haloperidol (HAL)-induced rats: induced rats were pre-, co- and post-treated with black seed oil (BSO), respectively, and the last group was treated with the oil only. The treatment was done through oral administration, and the experiment lasted 14 days.
Therapeutic administration of HAL to rats caused reduction in both enzymatic and non-enzymatic proteins mediated by stable OH˙ and DPPH free radicals. K+, Na+ and MDA contents as well as 51 nucleotidase, aldose-reductase activities were increased with corresponding decrease in the activity of lactate dehydrogenase (LDH) in HAL-induced toxicity rats. Contrariwise, differential treatments with BSO prevented and reversed the nephrotoxicity by depleting K+, Na+, MDA contents and aldose-reductase activity, and AMP hydrolysis with increased adenosine triphosphate (ATP) in the PMFs of rat kidney. The cytotoxicity of HAL elicited on both inner renal cortex and outer medulla was equally alleviated by combined active molecules of oil from black seed (OBS). However, pre-, co- and post-treatment demonstrate significant approaches in averting nephrotoxicity of neuroleptic drug (HAL) via several biological mechanisms.
This study therefore validates the use of black seed oil as therapy particularly for individuals with renal dysfunctions.
抗精神病药物可能对精神分裂症患者具有肾毒性。
本研究采用多种生物学方法，在成年大鼠中研究了黑种草籽油对肾功能障碍的保护作用。将动物分为六组（n = 10）：正常对照大鼠；氟哌啶醇（HAL）诱导的大鼠：诱导大鼠分别在预处理、共处理和后处理时使用黑种草籽油（BSO），最后一组仅用该油处理。通过口服给药进行治疗，实验持续14天。
给大鼠治疗性给予HAL导致由稳定的OH˙和DPPH自由基介导的酶性和非酶性蛋白质减少。在HAL诱导毒性的大鼠中，K +、Na +和丙二醛（MDA）含量以及5'-核苷酸酶、醛糖还原酶活性增加，同时乳酸脱氢酶（LDH）活性相应降低。相反，用BSO进行的不同处理通过消耗K +、Na +、MDA含量和醛糖还原酶活性以及AMP水解，同时增加大鼠肾脏质膜小泡中三磷酸腺苷（ATP），预防并逆转了肾毒性。黑种草籽油（OBS）中的活性分子组合同样减轻了HAL对内肾皮质和外髓质引起的细胞毒性。然而，预处理、共处理和后处理通过多种生物学机制，在避免抗精神病药物（HAL）的肾毒性方面显示出显著效果。
因此，本研究证实了黑种草籽油可作为一种治疗方法，尤其适用于肾功能障碍患者。
