Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Bioresour Technol. 2017 Jul;235:366-370. doi: 10.1016/j.biortech.2017.03.102. Epub 2017 Mar 22.
The high potential UV mutagenized Scenedesmus sp. was obtained in which the cells had a higher biomass and lipid content than the wild type with an increase from 1.9 to 2.4g/L and from 40 to 55% of dry cell weight respectively after 12days. Oxidative stress imposed by HO treatment decreased the biomass of both the wild type and the mutant. The HO treated mutant when grown in BG11 medium showed an increase in biomass which was in contrast to a decreased biomass observed in the HO treated wild type. A 3-fold increase in lipid yield of 1.63g/L was obtained in the oxidative stress-induced mutant compared to the wild type. Overall results indicate that prior treatment of UV-mutagenized Scenedesmus with oxidative stress can increase the total lipid production which, due to its derived methyl ester having acceptable biodiesel properties, can be potentially utilized for biodiesel production.
经高强度紫外线诱变选育出的栅藻（Scenedesmus sp.）具有更高的生物量和脂质含量，在 12 天内分别比野生型增加了 1.9 倍和 55%。HO 处理引起的氧化应激降低了野生型和突变型的生物量。在 BG11 培养基中生长的 HO 处理突变体显示生物量增加，而在 HO 处理的野生型中观察到生物量减少。与野生型相比，氧化应激诱导的突变体的脂质产率增加了 3 倍，达到 1.63g/L。总体结果表明，预先用氧化应激处理经紫外线诱变的栅藻可以增加总脂质产量，由于其衍生的甲酯具有可接受的生物柴油特性，因此可潜在用于生物柴油生产。
