University of Washington, Department of Bioengineering, Seattle, Washington, United StatesbTianjin University, College of Precision Instrument and Optoelectronics Engineering, Tianjin, China.
J Biomed Opt. 2017 Apr 1;22(4):46002. doi: 10.1117/1.JBO.22.4.046002.
We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.
我们提出了一种利用全场时变动态散斑信号测量毛细血管中红细胞（RBC）异速运动的方法。该方法利用低相干激光散斑成像系统记录瞬时散斑图案，然后使用基于特征分解的滤波算法提取由于运动 RBC 产生的动态散斑信号。通过对相邻位置获得的时间动态散斑信号进行互相关，可以确定异速 RBC 流的速度。我们通过对活体小鼠耳郭进行成像验证了该方法，证明了其具有全场 RBC 流映射和高分辨率量化流型的能力。预计该方法将用于研究生理变化下 RBC 在毛细血管中的动态作用。
