Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.
University of Chinese Academy of Sciences , Beijing 100049, China.
J Am Chem Soc. 2017 Apr 26;139(16):5877-5882. doi: 10.1021/jacs.7b01229. Epub 2017 Apr 14.
Zeolitic imidazole frameworks (ZIFs) are an emerging class of functional porous materials with promising biomedical applications such as molecular sensing and intracellular drug delivery. We report herein the first example of using nanoscale ZIFs (i.e., ZIF-90), self-assembled from Zn and imidazole-2-carboxyaldehyde, to target subcellular mitochondria and image dynamics of mitochondrial ATP in live cells. Encapsulation of fluorescent Rhodamine B (RhB) into ZIF-90 suppresses the emission of RhB, while the competitive coordination between ATP and the metal node of ZIF-90 dissembles ZIFs, resulting in the release of RhB for ATP sensing. With this method, we are able to image mitochondrial ATP in live cells and study the ATP level fluctuation in cellular glycolysis and apoptosis processes. The strategy reported here could be further extended to tune nanoscale ZIFs inside live cells for targeted delivery of therapeutics to subcellular organelles for advanced biomedical applications.
沸石咪唑酯骨架（ZIFs）是一类新兴的功能多孔材料，具有分子传感和细胞内药物输送等有前途的生物医学应用。本文首次报道了使用纳米级 ZIF-90（即由 Zn 和咪唑-2-甲醛自组装而成）靶向亚细胞线粒体并在活细胞中成像线粒体 ATP 的动态变化。将荧光 Rhodamine B（RhB）封装到 ZIF-90 中会抑制 RhB 的发射，而 ATP 与 ZIF-90 的金属节点之间的竞争配位会使 ZIF 解体，从而释放 RhB 以进行 ATP 传感。通过这种方法，我们能够在活细胞中成像线粒体 ATP，并研究细胞糖酵解和细胞凋亡过程中 ATP 水平的波动。本文报道的策略可以进一步扩展，以在活细胞内调节纳米级 ZIFs，用于靶向递送至亚细胞细胞器的治疗药物，以实现先进的生物医学应用。
