Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Biochemistry Laboratory, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Carbohydr Polym. 2017 Jun 15;166:228-235. doi: 10.1016/j.carbpol.2017.02.072. Epub 2017 Feb 22.
Hydroxyethyl chitosan (HECTS) is one of the most important water soluble derivatives of chitosan. In this study, photo-sensitive azidehydroxyethyl chitosan (AZ-HECTS) was synthesized with grafting degree 3.4%, and its water solution resulted in an insoluble hydrogel by 254nm UV irradiation for 90s. AZ-HECTS hydrogels, with water absorption 86.21%, had little impact on growth of mouse fibroblast (L929) and presented good cell biocompatibility. Obvious sudden degradation stage, slow degradation stage and no apparent toxicity was observed after AZ-HECTS hydrogels implanted into rats, and tissue inflammation was slighter, which indicated favorable biological safety. Furthermore, AZ-HECTS hydrogels was loaded with heparin, and released 50% of heparin accumulated 14d. AZ-HECTS-heparin hydrogels showed inhibitory effects on L929, and pro-growth functions within vascular endothelial cells seeded on the hydrogels, meanwhile a positive influence on vascular endothelial growth factor expression. In conclusion, AZ-HECTS hydrogels possessed favorable biocompatibility and biodegradability and had good potential as drug carrier.
羟乙基壳聚糖（HECTS）是壳聚糖最重要的水溶性衍生物之一。本研究用接枝率为 3.4%的方法合成了光敏感叠氮羟乙基壳聚糖（AZ-HECTS），其水溶液在 254nm 的紫外光照射 90 秒后会形成不溶性水凝胶。AZ-HECTS 水凝胶的吸水率为 86.21%，对小鼠成纤维细胞（L929）的生长几乎没有影响，表现出良好的细胞生物相容性。将 AZ-HECTS 水凝胶植入大鼠体内后，观察到明显的突然降解阶段、缓慢降解阶段和无明显毒性，组织炎症较轻，表明具有良好的生物安全性。此外，AZ-HECTS 水凝胶负载肝素，14d 后可释放 50%的累积肝素。AZ-HECTS-肝素水凝胶对接种在水凝胶上的血管内皮细胞的 L929 和促生长功能具有抑制作用，同时对血管内皮生长因子的表达也有积极影响。综上所述，AZ-HECTS 水凝胶具有良好的生物相容性和生物降解性，有望成为药物载体。
