School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
School of Pharmacy, Zunyi Medical College, Zunyi, Guizhou 563006, PR China.
Carbohydr Polym. 2017 Jun 15;166:358-364. doi: 10.1016/j.carbpol.2017.03.003. Epub 2017 Mar 4.
The use of cellulase hydrolysis of straw to produce fermentable sugars has many application prospects. However, cellulase is very expensive, which hampers its industrial applications. To improve cellulase's catalytic activity and reduce the enzyme cost, magnetite carboxymethyl chitosan/calcium alginate - cellulase bioconjugate (MCCCB) was synthesized via an improved hydrothermal method, molecular self-assembly technology, physical absorption, embedding and covalent bonding. Its loading capacity was 3.95mg/mL, and the catalytic activity increased to 267.18%. We decreased the release rate, improved the reusability, and enhanced the stability of MCCCB. Corn stalk hydrolysis also greatly improved, and the overall yield of fermentable sugars increased by 698.26%. All of these results indicate that MCCCB could significantly improve the efficiency of cellulase, greatly reduce the cost of enzyme, and effectively promote the production of fermentable sugars.
利用纤维素酶水解秸秆生产可发酵糖具有广阔的应用前景。然而，纤维素酶非常昂贵，这阻碍了它的工业应用。为了提高纤维素酶的催化活性并降低酶的成本，通过改进的水热法、分子自组装技术、物理吸附、包埋和共价键合合成了磁性羧甲基壳聚糖/海藻酸钠-纤维素酶生物偶联物（MCCCB）。其载药量为 3.95mg/mL，催化活性提高到 267.18%。我们降低了释放率，提高了可重复使用性，并增强了 MCCCB 的稳定性。玉米秸秆水解也得到了很大的改善，可发酵糖的总收率提高了 698.26%。所有这些结果表明，MCCCB 可以显著提高纤维素酶的效率，大大降低酶的成本，有效地促进可发酵糖的生产。
