人嗜T淋巴细胞病毒I型抗体检测方法的比较
Comparison of assays for antibody to HTLVI.
作者信息
White P M
机构信息
Virus Reference Laboratory, Central Public Health Laboratory, Colindale, London.
出版信息
J Clin Pathol. 1988 Jun;41(6):700-2. doi: 10.1136/jcp.41.6.700.
Indirect immunofluorescence, competitive radioimmunoassay, HTLV I-enzyme linked immunosorbent assay and gelatin particle agglutination Serodia-ATLA were compared in terms of their ability to detect antibody to human T cell leukaemia virus I (HTLV I). The sensitivities were 96.9%, 92%, 97.0%, and 100%, respectively, and the specificities 99.3%, 98.9%, 98.6%, and 96.3%. Particle agglutination was very simple to perform and was the most sensitive, though the least specific test. Antibody titres were 10-100 times higher when measured by particle agglutination than by other tests, and antibody titers were considerably higher in patients with neurological disease related to HTLV I than in those with other conditions. Serodia-ATLA is the method of choice for preliminary screening of specimens for antibody to HTLV I, but positive results must be confirmed by another technique.
对间接免疫荧光法、竞争性放射免疫测定法、人嗜T淋巴细胞病毒I型酶联免疫吸附测定法和明胶颗粒凝集血清学检测法（Serodia-ATLA）检测人嗜T淋巴细胞病毒I型（HTLV I）抗体的能力进行了比较。其灵敏度分别为96.9%、92%、97.0%和100%，特异性分别为99.3%、98.9%、98.6%和96.3%。颗粒凝集法操作非常简单，是最灵敏的检测方法，不过也是特异性最低的检测方法。通过颗粒凝集法测得的抗体滴度比其他检测方法高10至100倍，并且与HTLV I相关的神经疾病患者的抗体滴度比其他病症患者的抗体滴度高得多。血清学检测法（Serodia-ATLA）是对标本进行HTLV I抗体初步筛查的首选方法，但阳性结果必须通过另一种技术加以确认。
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