Nigro Ersilia, Scudiero Olga, Ludovica Monaco Maria, Polito Rita, Schettino Pietro, Grandone Anna, Perrone Laura, Miraglia Del Giudice Emanuele, Daniele Aurora
CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via A. Vivaldi 42, 81100 Caserta, Italy.
CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy.
Cytokine. 2017 Jun;94:8-13. doi: 10.1016/j.cyto.2016.12.018. Epub 2017 Apr 3.
Adiponectin (Acrp30), its high molecular weight (HMW) oligomers, and Irisin are molecules involved in several metabolic processes. To investigate if these cytokines could represent new metabolic markers, we evaluated the expression of Acrp30 and Irisin in serum of obese children from South Italy affected by different degrees of insulin resistance (IR). The anthropometric and metabolic features were evaluated in 27 obese children versus 13 age-matched controls. The expression of Acrp30, its pattern and Irisin were investigated by ELISA, western blotting and fast protein liquid chromatography. The HOMA index was significantly higher in obese children versus controls, and metabolic syndrome was more prevalent in obese children with elevated IR versus those with normal HOMA (38% vs 16%). Total Acrp30 and HMW oligomers were significantly lower in obese than in control children, and the difference was more pronounced in children with HOMA >3.4. In control and obese children, total Acrp30 and HMW oligomers were inversely related to HOMA (r-0.38, p 0.02; r-0.35, p 0.03). Irisin was significantly higher in obese than in control children, and was inversely correlated with Acrp30 and HMW (r-0.32, p 0.04; r-0.39, p 0.01). The inverse correlation of Acpr30 and HMW oligomers with HOMA indicates that Acpr30 is directly involved in IR status. Moreover, the inverse correlation between Irisin and Acrp30 and, more significantly, between Irisin and HMW oligomers suggests that the two cytokines are closely connected. The use of Acrp30, HMW oligomers and Irisin as predictive factors of IR in obese children remains to be further elucidated.
脂联素（Acrp30）、其高分子量（HMW）寡聚体和鸢尾素是参与多种代谢过程的分子。为了研究这些细胞因子是否可代表新的代谢标志物，我们评估了来自意大利南部不同程度胰岛素抵抗（IR）的肥胖儿童血清中Acrp30和鸢尾素的表达。对27名肥胖儿童和13名年龄匹配的对照者进行了人体测量和代谢特征评估。通过酶联免疫吸附测定（ELISA）、蛋白质印迹法和快速蛋白质液相色谱法研究了Acrp30的表达、其模式及鸢尾素。肥胖儿童的稳态模型评估（HOMA）指数显著高于对照者，且与正常HOMA的肥胖儿童相比，IR升高的肥胖儿童中代谢综合征更为普遍（38% 对16%）。肥胖儿童的总Acrp30和HMW寡聚体显著低于对照儿童，且在HOMA>3.4的儿童中差异更为明显。在对照儿童和肥胖儿童中，总Acrp30和HMW寡聚体与HOMA呈负相关（r=-0.38，p=0.02；r=-0.35，p=0.03）。肥胖儿童的鸢尾素显著高于对照儿童，且与Acrp30和HMW呈负相关（r=-0.32，p=0.04；r=-0.39，p=0.01）。Acrp30和HMW寡聚体与HOMA的负相关表明Acrp30直接参与IR状态。此外，鸢尾素与Acrp30之间以及更显著地与鸢尾素和HMW寡聚体之间的负相关表明这两种细胞因子密切相关。将Acrp30、HMW寡聚体和鸢尾素用作肥胖儿童IR预测因子的作用仍有待进一步阐明。
