Langeland N, Moore L J, Holmsen H, Haarr L
Department of Biochemistry, University of Bergen, Norway.
J Gen Virol. 1988 Jun;69 ( Pt 6):1137-45. doi: 10.1099/0022-1317-69-6-1137.
We earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (HSV-1) to the receptor of BHK cells, while the binding of HSV-2 to the receptor was unaffected. We could not determine whether the effect was on the virus particle, the receptor, or both. We have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of HSV-1 to the receptor by interfering with the cellular receptor function; higher molecular weight polylysines were more potent than those of lower molecular weight. Polylysine and neomycin showed additive effects. In vitro, polylysine showed the same strong binding to the plasma membrane phosphoinositides as did neomycin. Together these data suggest that the drugs may have a common target in the cell membrane. The HSV-1 and HSV-2 virus particles were unaffected by the drugs, as was the cellular HSV-2 receptor.
我们先前报道过，新霉素可可逆性地阻断单纯疱疹病毒1型（HSV-1）与BHK细胞受体的结合，而HSV-2与该受体的结合则不受影响。我们无法确定这种作用是针对病毒颗粒、受体，还是两者都有。我们现在测试了其他几种阳离子物质，并报告聚赖氨酸（和聚精氨酸）通过干扰细胞受体功能来阻断HSV-1与受体的结合；高分子量的聚赖氨酸比低分子量的更有效。聚赖氨酸和新霉素显示出相加作用。在体外，聚赖氨酸与质膜磷酸肌醇的结合与新霉素一样强。这些数据共同表明，这些药物可能在细胞膜上有共同的靶点。HSV-1和HSV-2病毒颗粒不受这些药物影响，细胞HSV-2受体也是如此。
