Binns M M, Tomley F M, Campbell J, Boursnell M E
Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, U.K.
J Gen Virol. 1988 Jun;69 ( Pt 6):1275-83. doi: 10.1099/0022-1317-69-6-1275.
The DNA sequence of a clustered set of genes which are conserved in orthopoxviruses has been determined for the avipoxvirus, fowlpox virus. The arrangement of the genes in fowlpox virus is nearly identical to that in vaccinia virus, and genes which are overlapping in vaccinia virus overlap in fowlpox virus. One major difference exists however, as the thymidine kinase (TK) gene is absent in fowlpox virus from the position it occupies within this cluster of genes in vaccinia virus. Instead, in fowlpox virus there is a 32 bp non-coding region present between the genes that flank the TK gene in vaccinia virus. The fowlpox virus TK gene has been cloned and sequenced. The sequences immediately flanking the TK gene show no homology to any previously reported poxvirus gene. These results are discussed in terms of genome stability in poxviruses and the use of the TK gene as a non-essential region for the introduction of foreign genes into poxviruses.
已确定禽痘病毒——鸡痘病毒中一组在正痘病毒中保守的成簇基因的DNA序列。鸡痘病毒中基因的排列与痘苗病毒几乎相同，痘苗病毒中重叠的基因在鸡痘病毒中也重叠。然而，存在一个主要差异，因为鸡痘病毒中不存在痘苗病毒该基因簇内胸腺嘧啶核苷激酶（TK）基因所占据位置的基因。相反，在鸡痘病毒中，痘苗病毒中位于TK基因两侧的基因之间存在一个32bp的非编码区。鸡痘病毒TK基因已被克隆并测序。TK基因两侧的序列与任何先前报道的痘病毒基因均无同源性。将根据痘病毒的基因组稳定性以及将TK基因用作向痘病毒中引入外源基因的非必需区域来讨论这些结果。
