Drillien R, Spehner D, Villeval D, Lecocq J P
Virology. 1987 Sep;160(1):203-9. doi: 10.1016/0042-6822(87)90061-4.
DNA from Fowlpox virus, a member of the Avipoxvirus genus, has been found to hybridize to DNA from vaccinia virus, a member of the Orthopoxvirus genus. The greatest homology detected was around the region containing the vaccinia virus thymidine kinase locus. A 3.1-kbp fowlpox virus fragment that hybridizes to the vaccinia virus HindIII J fragment has been cloned and its sequence determined. Comparison of the nucleotide and deduced amino acid sequence to the cross hybridizing vaccinia fragment revealed extensive conservation of six open reading frames as well as a similar organization along the genome. Nevertheless a fowlpox virus gene corresponding to the vaccinia virus thymidine kinase gene was apparently lacking within the region studied and is probably located elsewhere in the genome. Despite this intriguing divergence, our results indicate that the Avipoxviruses are more closely related to the Orthopoxviruses than previously suspected.
禽痘病毒属的成员禽痘病毒的DNA已被发现与正痘病毒属的成员痘苗病毒的DNA杂交。检测到的最大同源性位于包含痘苗病毒胸苷激酶基因座的区域周围。一个与痘苗病毒HindIII J片段杂交的3.1千碱基对禽痘病毒片段已被克隆并确定了其序列。将该核苷酸和推导的氨基酸序列与交叉杂交的痘苗片段进行比较，发现六个开放阅读框有广泛的保守性，并且沿基因组有相似的组织。然而，在所研究的区域内显然缺乏与痘苗病毒胸苷激酶基因相对应的禽痘病毒基因，该基因可能位于基因组的其他位置。尽管存在这种有趣的差异，但我们的结果表明，禽痘病毒与正痘病毒的关系比以前怀疑的更为密切。
