Tymoshenko Alexey, Tkach Gennadii, Sikora Vitalii, Bumeister Valentina, Shpetnyi Ihor, Lyndin Mykola, Maksymova Olena, Maslenko Anna
Department of Normal Anatomy with a Topographical Anatomy and Operational Surgery Course, Sumy State University , Sumy, Ukraine.
Department of Applied Physics, Sumy State University , Sumy, Ukraine.
Interv Med Appl Sci. 2016 Jun 1;8(2):82-88. doi: 10.1556/1646.8.2016.2.7.
The article is devoted to study the structural changes in the skeletal muscles caused by heavy metal salts.
The study was conducted on 72 mature male rats. The experimental groups were given to drink water with combinations of heavy metal salts for one, two and three months. This type of water is typical for the water basins in the northern districts of the Sumy region. The study of morphological changes in the striated muscles was concluded using light and scanning electron microscopy.
The data analysis revealed that a prolonged duration of negative factor could intensify sclerotic and edematous processes. The structure of muscle fibers was destroyed, nuclei were deformed and placed irregularly, and many petechial hemorrhages occurred. Besides, cross-striation was irregular, I and A bands were deformed and destroyed, H band was hardly visualized. The inner mitochondrial membrane and cristae become deformed. The symplastic nuclei were placed irregularly within sarcoplasm. Besides, they were swollen. Against swollen and enlarged symplastic nuclei, pyknotic nuclei were also found. The structures of sarcoplasmic reticulum were mainly dilated with deformed and ruptured areas.
Our study approves that high concentrations of heavy metal salts have a destructive influence on the skeletal striated muscles.
本文致力于研究重金属盐对骨骼肌结构的影响。
对72只成年雄性大鼠进行研究。实验分组给予含重金属盐组合的饮水，为期1个月、2个月和3个月。这种水是苏梅州北部地区水域的典型水质。使用光学显微镜和扫描电子显微镜对横纹肌的形态变化进行研究。
数据分析表明，负面因素的长期作用会加剧硬化和水肿过程。肌纤维结构遭到破坏，细胞核变形且分布不规则，出现多处点状出血。此外，横纹不规则，I带和A带变形并遭到破坏，H带几乎不可见。线粒体内膜和嵴变形。合胞核在肌浆内分布不规则，且肿胀。除了肿胀和增大的合胞核外，还发现了固缩核。肌浆网结构主要是扩张，伴有变形和破裂区域。
我们的研究证实，高浓度重金属盐对骨骼肌有破坏作用。
