Wongsupa Natkrita, Nuntanaranont Thongchai, Kamolmattayakul Suttatip, Thuaksuban Nuttawut
Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
J Mater Sci Mater Med. 2017 May;28(5):77. doi: 10.1007/s10856-017-5883-x. Epub 2017 Apr 6.
The objective of the present study was to investigate the effect of a fabricated combination of poly-ɛ-caprolactone (PCL)-biphasic calcium phosphate (BCP) with the modified melt stretching and multilayer deposition (mMSMD) technique on human dental pulp stem cell (hDPSC) differentiation to be osteogenic like cells for bone regeneration of calvarial defects in rabbit models. hDPSCs extracted from human third molars were seeded onto mMSMD PCL-BCP scaffolds and the osteogenic gene expression was tested prior to implantation in vivo. Two standardized 11 mm in diameter circular calvarial defects were created in 18 adult male New Zealand white rabbits. The rabbits were divided into 4 groups: (1) hDPSCs seeded in mMSMD PCL-BCP scaffolds; (2) mMSMD PCL-BCP scaffolds alone, (3) empty defects and (4) autogenous bone (n = 3 site/time point/groups). After two, four and eight weeks after the operation, the specimens were harvested for micro-CT including histological and histomorphometric analysis. The explicit results presented an interesting view of the bioengineered constructs of hDPSCs in PCL-BCP scaffolds that increased the newly formed bone compared to the empty defect and scaffold alone groups. The results demonstrated that hDPSCs combined with mMSMD PCL-BCP scaffolds may be an augmentation material for bony defect.
本研究的目的是研究采用改良熔体拉伸和多层沉积（mMSMD）技术制备的聚-ε-己内酯（PCL）-双相磷酸钙（BCP）组合对人牙髓干细胞（hDPSC）分化为成骨样细胞以促进兔模型颅骨缺损骨再生的影响。从人类第三磨牙中提取的hDPSC接种到mMSMD PCL-BCP支架上，并在体内植入前测试其成骨基因表达。在18只成年雄性新西兰白兔中制造两个直径为11毫米的标准化圆形颅骨缺损。将兔子分为4组：（1）接种在mMSMD PCL-BCP支架上的hDPSC；（2）单独的mMSMD PCL-BCP支架；（3）空白缺损；（4）自体骨（每组/每个时间点n = 3个部位）。术后两周、四周和八周，采集标本进行显微CT检查，包括组织学和组织形态计量学分析。明确的结果显示了PCL-BCP支架中hDPSC生物工程构建体的有趣情况，与空白缺损组和单独支架组相比，其新形成的骨增加。结果表明，hDPSC与mMSMD PCL-BCP支架结合可能是一种用于骨缺损的增强材料。
