Supphaprasitt Woraporn, Charoenmuang Lalita, Thuaksuban Nuttawut, Sangsuwan Prawichaya, Leepong Narit, Supakanjanakanti Danaiya, Vongvatcharanon Surapong, Suwanrat Trin, Srimanok Woraluk
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai 90110, Thailand.
Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai 90110, Thailand.
J Funct Biomater. 2022 Jul 15;13(3):93. doi: 10.3390/jfb13030093.
The efficacy of a three-dimensional printed polycaprolactone-biphasic-calcium-phosphate scaffold (PCL-BCP TDP scaffold) seeded with adipose-derived stem cells (ADSCs), which were cultured in xenogeneic serum-free media (XSFM) to enhance bone formation, was assessed in vitro and in animal models. The ADSCs were isolated from the buccal fat tissue of six patients using enzymatic digestion and the plastic adherence method. The proliferation and osteogenic differentiation of the cells cultured in XSFM when seeded on the scaffolds were assessed and compared with those of cells cultured in a medium containing fetal bovine serum (FBS). The cell-scaffold constructs were cultured in XSFM and were implanted into calvarial defects in thirty-six Wistar rats to assess new bone regeneration. The proliferation and osteogenic differentiation of the cells in the XSFM medium were notably better than that of the cells in the FBS medium. However, the efficacy of the constructs in enhancing new bone formation in the calvarial defects of rats was not statistically different to that achieved using the scaffolds alone. In conclusion, the PCL-BCP TDP scaffolds were biocompatible and suitable for use as an osteoconductive framework. The XSFM medium could support the proliferation and differentiation of ADSCs in vitro. However, the cell-scaffold constructs had no benefit in the enhancement of new bone formation in animal models.
评估了接种脂肪来源干细胞（ADSCs）的三维打印聚己内酯 - 双相磷酸钙支架（PCL - BCP TDP支架）的功效，这些细胞在异种无血清培养基（XSFM）中培养以促进骨形成，并在体外和动物模型中进行了评估。通过酶消化和塑料贴壁法从六名患者的颊脂肪组织中分离出ADSCs。评估了接种在支架上的细胞在XSFM中培养时的增殖和成骨分化情况，并与在含有胎牛血清（FBS）的培养基中培养的细胞进行了比较。将细胞 - 支架构建体在XSFM中培养，并植入36只Wistar大鼠的颅骨缺损处，以评估新骨再生情况。XSFM培养基中细胞的增殖和成骨分化明显优于FBS培养基中的细胞。然而，构建体在增强大鼠颅骨缺损处新骨形成方面的功效与单独使用支架所达到的功效在统计学上没有差异。总之，PCL - BCP TDP支架具有生物相容性，适合用作骨传导框架。XSFM培养基可以在体外支持ADSCs的增殖和分化。然而，细胞 - 支架构建体在动物模型中对增强新骨形成没有益处。
