DNA凝胶电泳中条带的混乱。

Lalande M, Noolandi J, Turmel C, Brousseau R, Rousseau J, Slater G W

National Research Council Canada, Biotechnology Research Institute, Montréal, Québec.

Nucleic Acids Res. 1988 Jun 24;16(12):5427-37. doi: 10.1093/nar/16.12.5427.

Under certain conditions of agarose gel electrophoresis, larger DNA molecules migrate faster than smaller ones. This anomalous mobility of DNA, which can lead to serious errors in the measurement of DNA fragment lengths, is related to near-zero velocity conformations which can trap DNA chains during electrophoresis. Intermittent electric fields can be used to alter the chain conformations so as to restore the monotonic mobility-size relationship which is necessary for a correct interpretation of the gel. These data are in agreement with the results of a computer simulation based on a theoretical model of electrophoresis.

在某些琼脂糖凝胶电泳条件下，较大的DNA分子比较小的DNA分子迁移得更快。DNA的这种异常迁移率可能会导致DNA片段长度测量中出现严重误差，这与接近零速度的构象有关，这种构象在电泳过程中会捕获DNA链。可以使用间歇电场来改变链构象，以恢复凝胶正确解读所必需的单调迁移率-大小关系。这些数据与基于电泳理论模型的计算机模拟结果一致。