Elder J K, Amos A, Southern E M, Shippey G A
Anal Biochem. 1983 Jan;128(1):223-6. doi: 10.1016/0003-2697(83)90368-8.
The electrophoretic mobilities of DNA polymer fragments in an agarose gel have been measured from a photograph of the gel by different methods and converted to lengths by the reciprocal method. The method of measurement can introduce large errors in the length estimates. The use of a digital microdensitometer to obtain optical density profiles of gel tracks with subsequent computer processing to find peak positions was found to give the most accurate DNA lengths.
通过不同方法从琼脂糖凝胶的照片中测量了DNA聚合物片段在凝胶中的电泳迁移率，并通过倒数法将其转换为长度。测量方法可能会在长度估计中引入较大误差。使用数字显微密度计获取凝胶条带的光密度分布图，随后进行计算机处理以找到峰位置，被发现能给出最准确的DNA长度。
