有色大鼠和白化大鼠对中度、急性和可逆性眼内压升高的反应有所不同。
Pigmented and albino rats differ in their responses to moderate, acute and reversible intraocular pressure elevation.
作者信息
Gurdita Akshay, Tan Bingyao, Joos Karen M, Bizheva Kostadinka, Choh Vivian
机构信息
School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
Department of Physics and Astronomy, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.
出版信息
Doc Ophthalmol. 2017 Jun;134(3):205-219. doi: 10.1007/s10633-017-9586-x. Epub 2017 Apr 7.
PURPOSE
To compare the electrophysiological and morphological responses to acute, moderately elevated intraocular pressure (IOP) in Sprague-Dawley (SD), Long-Evans (LE) and Brown Norway (BN) rat eyes.
METHODS
Eleven-week-old SD (n = 5), LE (n = 5) and BN (n = 5) rats were used. Scotopic threshold responses (STRs), Maxwellian flash electroretinograms (ERGs) or ultrahigh-resolution optical coherence tomography (UHR-OCT) images of the rat retinas were collected from both eyes before, during and after IOP elevation of one eye. IOP was raised to ~35 mmHg for 1 h using a vascular loop, while the other eye served as a control. STRs, ERGs and UHR-OCT images were acquired on 3 days separated by 1 day of no experimental manipulation.
RESULTS
There were no significant differences between species in baseline electroretinography. However, during IOP elevation, peak positive STR amplitudes in LE (mean ± standard deviation 259 ± 124 µV) and BN (228 ± 96 µV) rats were about fourfold higher than those in SD rats (56 ± 46 µV) rats (p = 0.0002 for both). Similarly, during elevated IOP, ERG b-wave amplitudes were twofold higher in LE and BN rats compared to those of SD rats (947 ± 129 µV and 892 ± 184 µV, vs 427 ± 138 µV; p = 0.0002 for both). UHR-OCT images showed backward bowing in all groups during IOP elevation, with a return to typical form about 30 min after IOP elevation.
CONCLUSION
Differences in the loop-induced responses between the strains are likely due to different inherent retinal morphology and physiology.
目的
比较斯普拉格-道利（SD）大鼠、长-伊文斯（LE）大鼠和挪威棕（BN）大鼠眼睛对急性、中度升高眼压（IOP）的电生理和形态学反应。
方法
使用11周龄的SD大鼠（n = 5）、LE大鼠（n = 5）和BN大鼠（n = 5）。在一只眼睛眼压升高前、升高期间和升高后，从两只眼睛收集大鼠视网膜的暗视阈值反应（STRs）、马克斯韦尔闪光视网膜电图（ERGs）或超高分辨率光学相干断层扫描（UHR-OCT）图像。使用血管环将眼压升高至约35 mmHg并持续1小时，而另一只眼睛作为对照。在3天内采集STRs、ERGs和UHR-OCT图像，中间间隔1天不进行实验操作。
结果
各物种之间基线视网膜电图无显著差异。然而，在眼压升高期间，LE大鼠（平均值±标准差 259 ± 124 μV）和BN大鼠（228 ± 96 μV）的STR正向峰值幅度比SD大鼠（56 ± 46 μV）高约四倍（两者p = 0.0002）。同样，在眼压升高期间，LE大鼠和BN大鼠的ERG b波幅度比SD大鼠高两倍（947 ± 129 μV和892 ± 184 μV，对比427 ± 138 μV；两者p = 0.0002）。UHR-OCT图像显示，在眼压升高期间所有组均出现向后弯曲，眼压升高后约30分钟恢复为典型形态。
结论
不同品系之间环诱导反应的差异可能是由于视网膜固有形态和生理的不同。
Zotero 插件