拟南芥质膜的蛋白质组学分析揭示了脂多糖反应性变化。

Proteomic analysis of Arabidopsis plasma membranes reveals lipopolysaccharide-responsive changes.

作者信息

Baloyi Nwaxigombe M, Dubery Ian A, Piater Lizelle A

机构信息

Department of Biochemistry, University of Johannesburg, Auckland Park, Johannesburg, South Africa.

出版信息

Biochem Biophys Res Commun. 2017 May 13;486(4):1137-1142. doi: 10.1016/j.bbrc.2017.04.016. Epub 2017 Apr 6.

DOI:10.1016/j.bbrc.2017.04.016

PMID:28390899

Abstract

Plant plasma membranes (PMs) contain pattern recognition receptors (PRRs), lately believed to be associated within multicomponent complexes, which perceive microbe-associated molecular pattern (MAMP) molecules like lipopolysaccharides (LPSs) and result in signal transduction events that lead to activated immune defense responses. As such, Arabidopsis thaliana leaves were treated with LPS from Escherichia coli (LPS) over time, and PM fractions isolated and evaluated using gel-based and subsequent mass spectrometry approaches for identification of LPS-responsive proteins. From the identified protein bands and spots, it is concluded that perception of hexaacylated LPS and resulting signal transduction occurs via PM-associated protein(s), amongst others, receptor-like kinases (RLKs) including G-type lectin S-receptor kinase and BAK1, and mostly likely within specialized perception domains.

摘要

植物质膜（PMs）含有模式识别受体（PRRs），近来认为其与多组分复合物相关，该复合物能识别诸如脂多糖（LPSs）等微生物相关分子模式（MAMP）分子，并引发信号转导事件，进而导致激活的免疫防御反应。因此，随着时间推移，用来自大肠杆菌的LPS（LPS）处理拟南芥叶片，并分离质膜组分，使用基于凝胶的方法及后续质谱方法评估，以鉴定LPS反应蛋白。从鉴定出的蛋白条带和斑点可以得出结论，六酰化LPS的识别及由此产生的信号转导是通过与质膜相关的蛋白质发生的，其中包括受体样激酶（RLKs），如G型凝集素S受体激酶和BAK1，并且很可能发生在特定的识别区域内。