Santos Marta, Dias-Pereira Patrícia, Williams Christina, Lopes Carlos, Breen Matthew
Department of Microscopy, Laboratory of Histology and Embryology, Institute of Biomedical Sciences Abel Salazar, ICBAS - UPorto, University of Porto, Porto, Portugal.
Department of Pathology and Molecular Immunology, ICBAS - UPorto, Porto, Portugal.
Vet J. 2017 Apr;222:68-71. doi: 10.1016/j.tvjl.2017.03.005. Epub 2017 Mar 28.
Neoplastic mammary disease in female dogs represents a major health concern for dog owners and veterinarians, but the genomic basis of the disease is poorly understood. In this study, we performed high resolution oligonucleotide array comparative genomic hybridisation (oaCGH) to assess genome wide DNA copy number changes in 10 malignant canine mammary tumours from seven female dogs, including multiple tumours collected at one time from each of three female dogs. In all but two tumours, genomic imbalances were detected, with losses being more common than gains. Canine chromosomes 9, 22, 26, 27, 34 and X were most frequently affected. Dissimilar oaCGH ratio profiles were observed in multiple tumours from the same dogs, providing preliminary evidence for probable independent pathogenesis. Analysis of adjacent samples of one tumour revealed regional differences in the number of genomic imbalances, suggesting heterogeneity within tumours.
雌性犬类的肿瘤性乳腺疾病是犬主和兽医主要关注的健康问题，但对该疾病的基因组基础了解甚少。在本研究中，我们进行了高分辨率寡核苷酸阵列比较基因组杂交（oaCGH），以评估来自7只雌性犬的10个恶性犬乳腺肿瘤的全基因组DNA拷贝数变化，其中包括从3只雌性犬中每只一次采集的多个肿瘤。除两个肿瘤外，在所有肿瘤中均检测到基因组失衡，缺失比增加更为常见。犬的9号、22号、26号、27号、34号染色体和X染色体受影响最为频繁。在同一只犬的多个肿瘤中观察到不同的oaCGH比率图谱，为可能的独立发病机制提供了初步证据。对一个肿瘤的相邻样本分析显示，基因组失衡数量存在区域差异，表明肿瘤内部存在异质性。
