Beesoo Rima, Bhagooli Ranjeet, Neergheen-Bhujun Vidushi S, Li Wen-Wu, Kagansky Alexander, Bahorun Theeshan
Department of Biosciences, Faculty of Science, University of Mauritius, Réduit 80837, Republic of Mauritius; ANDI Centre of Excellence for Biomedical and Biomaterials Research, MSIRI Building, University of Mauritius, Réduit 80837, Republic of Mauritius; Department of Health Sciences, Faculty of Science University of Mauritius, Réduit, Republic of Mauritius.
Department of Marine and Ocean Science, Fisheries and Mariculture, Faculty of Ocean Studies, University of Mauritius, Réduit 80837, Republic of Mauritius.
Comp Biochem Physiol C Toxicol Pharmacol. 2017 Jun;196:81-90. doi: 10.1016/j.cbpc.2017.04.001. Epub 2017 Apr 7.
Increasing prevalence of antibiotic resistance has led research to focus on discovering new antimicrobial agents derived from the marine biome. Although ample studies have investigated sponges for their bioactive metabolites with promising prospects in drug discovery, the potentiating effects of sponge extracts on antibiotics still remains to be expounded. The present study aimed to investigate the antibacterial capacity of seven tropical sponges collected from Mauritian waters and their modulatory effect in association with three conventional antibiotics namely chloramphenicol, ampicillin and tetracycline. Disc diffusion assay was used to determine the inhibition zone diameter (IZD) of the sponge total crude extracts (CE), hexane (HF), ethyl acetate (EAF) and aqueous (AF) fractions against nine standard bacterial isolates whereas broth microdilution method was used to determine their minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs) and antibiotic potentiating activity of the most active sponge extract. MIC values of the sponge extracts ranged from 0.039 to 1.25mg/mL. Extracts from Neopetrosia exigua rich in beta-sitosterol and cholesterol displayed the widest activity spectrum against the 9 tested bacterial isolates whilst the best antibacterial profile was observed by its EAF particularly against Staphylococcus aureus and Bacillus cereus with MIC and MBC values of 0.039mg/mL and 0.078mg/mL, respectively. The greatest antibiotic potentiating effect was obtained with the EAF of N. exigua (MIC/2) and ampicillin combination against S. aureus. These findings suggest that the antibacterial properties of the tested marine sponge extracts may provide an alternative and complementary strategy to manage bacterial infections.
抗生素耐药性的日益普遍促使研究聚焦于发现源自海洋生物群落的新型抗菌剂。尽管已有大量研究调查海绵的生物活性代谢产物在药物发现方面具有广阔前景，但海绵提取物对抗生素的增效作用仍有待阐明。本研究旨在调查从毛里求斯海域采集的七种热带海绵的抗菌能力及其与三种传统抗生素（即氯霉素、氨苄青霉素和四环素）联合使用时的调节作用。采用纸片扩散法测定海绵总粗提物（CE）、己烷馏分（HF）、乙酸乙酯馏分（EAF）和水相馏分（AF）对九种标准细菌分离株的抑菌圈直径（IZD），而采用肉汤微量稀释法测定其最低抑菌浓度（MIC）、最低杀菌浓度（MBC）以及活性最强的海绵提取物的抗生素增效活性。海绵提取物的MIC值范围为0.039至1.25mg/mL。富含β-谷甾醇和胆固醇的微小新岩海绵提取物对9种受试细菌分离株显示出最广的活性谱，而其EAF表现出最佳的抗菌谱，尤其是对金黄色葡萄球菌和蜡样芽孢杆菌，MIC和MBC值分别为0.039mg/mL和0.078mg/mL。微小新岩海绵的EAF（MIC/2）与氨苄青霉素联合使用对金黄色葡萄球菌产生了最大的抗生素增效作用。这些发现表明，受试海洋海绵提取物的抗菌特性可能为控制细菌感染提供一种替代和补充策略。
