Pak J Biol Sci. 2020 Jan;23(11):1436-1441. doi: 10.3923/pjbs.2020.1436.1441.
The urgent of finding new antibiotics due to the rising of antibiotic-resistant bacteria. The plant is the main source of new antibiotic substances. The purpose of this research was to evaluate the antibacterial activity of Spathiphyllum wallisii extracts against nine human pathogenic bacteria.
The stalks, leaf, rhizome and root of S. wallisii were extracted by using hexane, dichloromethane, ethyl acetate, ethanol and methanol. The disc diffusion assay was used to screen the antibacterial activity of S. wallisii extracts. Broth dilution and colorimetric assay were used to determine the Minimal inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values of extracts.
The lowest MIC values at 0.048 mg mL-1 were presented in the stalks extract with dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008, the leaf extracted with hexane, dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008; the leaf extracted with ethyl acetate, methanol and ethanol against S. aureus TISTR 1466, the leaf extracted with dichloromethane, ethyl acetate, methanol and ethanol against S. aureus PK; the rhizome extracted with methanol against S. aureus PK. The lowest of MBC value of 0.048 mg mL-1 was obtained from methanolic rhizome extract against B. subtilis TISTR 008.
The methanolic rhizome extract of S. wallisii demonstrated the highest of pathogenic bacterial growth inhibition. This is the first report about the antibacterial activity of S. wallisii extracts that will add new information in natural drug discovery and development in industrial pharmacology.
由于抗生素耐药菌的出现，寻找新抗生素变得迫在眉睫。植物是新抗生素物质的主要来源。本研究旨在评估石蒜鳞茎提取物对九种人体致病细菌的抗菌活性。
采用正己烷、二氯甲烷、乙酸乙酯、乙醇和甲醇对石蒜鳞茎的茎、叶、根茎和根进行提取。采用圆盘扩散法筛选石蒜鳞茎提取物的抗菌活性。采用肉汤稀释和比色法测定提取物的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）值。
二氯甲烷、乙酸乙酯、甲醇和乙醇提取物对枯草芽孢杆菌 TISTR 008 的 MIC 值最低，为 0.048mg/mL-1，正己烷、二氯甲烷、乙酸乙酯、甲醇和乙醇提取物对枯草芽孢杆菌 TISTR 008 的 MIC 值最低；乙酸乙酯、甲醇和乙醇提取物对金黄色葡萄球菌 TISTR 1466 的 MIC 值最低，二氯甲烷、乙酸乙酯、甲醇和乙醇提取物对金黄色葡萄球菌 PK 的 MIC 值最低；甲醇提取物对金黄色葡萄球菌 PK 的 MIC 值最低，为 0.048mg/mL-1。甲醇提取物对枯草芽孢杆菌 TISTR 008 的 MBC 值最低，为 0.048mg/mL-1。
石蒜鳞茎的甲醇提取物对病原菌的生长抑制作用最强。这是首次报道石蒜鳞茎提取物的抗菌活性，将为天然药物发现和工业药理学的发展提供新的信息。
