Shirazi Mohsen, Vaziri Hamed, Salari Behzad, Motahhari Pouria, Etemad-Moghadam Shahroo, Dehpour Ahmad Reza
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Orthodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Private practice, Houston, Texas.
Iran J Basic Med Sci. 2017 Mar;20(3):260-264. doi: 10.22038/ijbms.2017.8353.
to determine the effect of different doses of caffeine on orthodontic tooth movement (OTM) in rats.
Forty male 250-300 g Sprague-Dawley rats were randomly divided into four groups of ten animals each and received 0 (control), 1 g/l, 2 g/l and 3 g/l caffeine in tap water for 3 days. Orthodontic appliances were ligated between the maxillary first molars and incisors on the 4th day of the study period. All rats were sacrificed after 2 weeks of treatment after which OTM was measured. Hematoxylin/eosin-stained sections of the molars were prepared and the mesial roots were examined for resorption-lacunae depth and osteoclast number. ANOVA was used for statistical analysis (<0.05).
A significant decrease in OTM was observed only in the 2 g/l (=0.043) and 3 g/l (<0.01) caffeine-receiving rats compared to the control animals. Osteoclast counts and resorption-lacunae depths demonstrated significant differences between each of the caffeine groups and control rats (<0.05). None of the variables showed significant differences between the caffeine groups (>0.05).
According to our findings, one of the effects of caffeine consumption during orthodontic treatment in rats was decreased root resorption. Additionally, concentrations of 2 g/l and 3 g/l inhibited OTM which seems to be due to its influence on osteoclast numbers.
确定不同剂量咖啡因对大鼠正畸牙齿移动（OTM）的影响。
将40只体重250 - 300克的雄性斯普拉格 - 道利大鼠随机分为四组，每组10只，分别饮用含0（对照组）、1克/升、2克/升和3克/升咖啡因的自来水，持续3天。在研究期第4天，在上颌第一磨牙和切牙之间结扎正畸矫治器。治疗2周后处死所有大鼠，然后测量OTM。制备磨牙苏木精/伊红染色切片，检查近中牙根的吸收陷窝深度和破骨细胞数量。采用方差分析进行统计分析（<0.05）。
与对照动物相比，仅在饮用2克/升（=0.043）和3克/升（<0.01）咖啡因的大鼠中观察到OTM显著降低。各咖啡因组与对照大鼠之间的破骨细胞计数和吸收陷窝深度存在显著差异（<0.05）。咖啡因组之间的所有变量均未显示出显著差异（>0.05）。
根据我们的研究结果，大鼠正畸治疗期间摄入咖啡因的影响之一是牙根吸收减少。此外，2克/升和3克/升的浓度抑制了OTM，这似乎是由于其对破骨细胞数量的影响。
