Hajihoseini Niloofar, Rezvani Gita, Etemad-Moghadam Shahroo
Department of Oral and Maxillofacial Pathology, Shahed University, Tehran, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Dent Res J (Isfahan). 2020 Dec 10;17(6):452-458. eCollection 2020 Nov-Dec.
The aim of this study was to compare conventional and microwave-assisted decalcification of sheep bone with and without teeth and to detect any difference in tissue detail preservation, staining quality, and rate of decalcification.
In this method analysis study, twenty-four specimens consisting of 12 blocks of mandibular molars with their surrounding bone and 12 blocks of mandibular osseous tissue were allocated into two microwave or routine decalcification groups using 5% nitric or formic acid as decalcifying agents. In addition to decalcification rate, a number of variables were used to assess staining quality and tissue detail preservation which were compared between the two groups using Mann-Whitney test ( < 0.05).
Time to complete decalcification was significantly reduced in the microwave-treated samples as compared to the conventional method, regardless of the decalcifying agent ( = 0.025). For both acids, most variables related to staining quality and tissue detail preservation were similar between the techniques ( > 0.05). Patchy staining in bone samples and tissue tears in bone + teeth specimens were more common in the routine method when using nitric acid (NAc) ( = 0.046) and formic acid (FAc) ( = 0.046), respectively. In comparing acids, the performance of FAc was slightly inferior to that of NAc, especially for specimens containing both tooth and bone.
The use of microwave technology can accelerate decalcification of bone and teeth of sheep mandibles and at the same time preserve tissue structure and staining quality. Further studies are required to help select the best demineralizing agent, especially in specimens containing bone and teeth.
本研究旨在比较有牙和无牙绵羊骨的传统脱钙和微波辅助脱钙，并检测组织细节保存、染色质量和脱钙速率方面的差异。
在本方法分析研究中，将24个标本（包括12块下颌磨牙及其周围骨和12块下颌骨组织）分为两个微波或常规脱钙组，使用5%硝酸或甲酸作为脱钙剂。除脱钙速率外，还使用多个变量评估染色质量和组织细节保存情况，并使用Mann-Whitney检验（<0.05）在两组之间进行比较。
与传统方法相比，无论使用何种脱钙剂，微波处理样本完成脱钙的时间均显著缩短（=0.025）。对于两种酸，两种技术在与染色质量和组织细节保存相关的大多数变量上相似（>0.05）。使用硝酸（NAc）（=0.046）和甲酸（FAc）（=0.046）时，常规方法中骨样本的斑驳染色和骨+牙标本中的组织撕裂分别更为常见。在比较酸时，FAc的性能略逊于NAc，尤其是对于同时含有牙和骨的标本。
微波技术的使用可加速绵羊下颌骨的骨和牙脱钙，同时保留组织结构和染色质量。需要进一步研究以帮助选择最佳脱钙剂，特别是在含有骨和牙的标本中。
